View source for 7b23


==DtxR-like iron-dependent regulator IdeR complexed with cobalt and the SACE_2689 promoter DNA-binding sequence==
<StructureSection load='7b23' size='340' side='right'caption='[[7b23]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7b23]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharopolyspora_erythraea_NRRL_2338 Saccharopolyspora erythraea NRRL 2338] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B23 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b23 OCA], [https://pdbe.org/7b23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b23 RCSB], [https://www.ebi.ac.uk/pdbsum/7b23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b23 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The iron-dependent regulator IdeR is the main transcriptional regulator controlling iron homeostasis genes in Actinobacteria, including species from the Corynebacterium, Mycobacterium and Streptomyces genera, as well as the erythromycin-producing bacterium Saccharopolyspora erythraea. Despite being a well-studied transcription factor since the identification of the Diphtheria toxin repressor DtxR three decades ago, the details of how IdeR proteins recognize their highly conserved 19-bp DNA target remain to be elucidated. IdeR makes few direct contacts with DNA bases in its target sequence, and we show here that these contacts are not required for target recognition. The results of our structural and mutational studies support a model wherein IdeR mainly uses an indirect readout mechanism, identifying its targets via the sequence-dependent DNA backbone structure rather than through specific contacts with the DNA bases. Furthermore, we show that IdeR efficiently recognizes a shorter palindromic sequence corresponding to a half binding site as compared to the full 19-bp target previously reported, expanding the number of potential target genes controlled by IdeR proteins.

The bacterial iron sensor IdeR recognizes its DNA targets by indirect readout.,Marcos-Torres FJ, Maurer D, Juniar L, Griese JJ Nucleic Acids Res. 2021 Sep 27;49(17):10120-10135. doi: 10.1093/nar/gkab711. PMID:34417623<ref>PMID:34417623</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7b23" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharopolyspora erythraea NRRL 2338]]
[[Category: Synthetic construct]]
[[Category: Griese JJ]]
[[Category: Marcos-Torres FJ]]
[[Category: Maurer D]]