View source for 7bax


==Crystal structure of LYS11 ectodomain==
<StructureSection load='7bax' size='340' side='right'caption='[[7bax]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7bax]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lotus_japonicus Lotus japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BAX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BAX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bax FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bax OCA], [https://pdbe.org/7bax PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bax RCSB], [https://www.ebi.ac.uk/pdbsum/7bax PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bax ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D3KTZ8_LOTJA D3KTZ8_LOTJA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Plants and animals use cell surface receptors to sense and interpret environmental signals. In legume symbiosis with nitrogen-fixing bacteria, the specific recognition of bacterial lipochitooligosaccharide (LCO) signals by single-pass transmembrane receptor kinases determines compatibility. Here, we determine the structural basis for LCO perception from the crystal structures of two lysin motif receptor ectodomains and identify a hydrophobic patch in the binding site essential for LCO recognition and symbiotic function. We show that the receptor monitors the composition of the amphiphilic LCO molecules and uses kinetic proofreading to control receptor activation and signaling specificity. We demonstrate engineering of the LCO binding site to fine-tune ligand selectivity and correct binding kinetics required for activation of symbiotic signaling in plants. Finally, the hydrophobic patch is found to be a conserved structural signature in this class of LCO receptors across legumes that can be used for in silico predictions. Our results provide insights into the mechanism of cell-surface receptor activation by kinetic proofreading of ligands and highlight the potential in receptor engineering to capture benefits in plant-microbe interactions.

Kinetic proofreading of lipochitooligosaccharides determines signal activation of symbiotic plant receptors.,Gysel K, Laursen M, Thygesen MB, Lironi D, Bozsoki Z, Hjuler CT, Maolanon NN, Cheng J, Bjork PK, Vinther M, Madsen LH, Rubsam H, Muszynski A, Ghodrati A, Azadi P, Sullivan JT, Ronson CW, Jensen KJ, Blaise M, Radutoiu S, Stougaard J, Andersen KR Proc Natl Acad Sci U S A. 2021 Nov 2;118(44). pii: 2111031118. doi:, 10.1073/pnas.2111031118. PMID:34716271<ref>PMID:34716271</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7bax" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Lotus japonicus]]
[[Category: Andersen KR]]
[[Category: Blaise M]]
[[Category: Cheng J]]
[[Category: Gysel K]]
[[Category: Laursen M]]