View source for 7bdv


==Structure of Can2 from Sulfobacillus thermosulfidooxidans in complex with cyclic tetra-adenylate (cA4)==
<StructureSection load='7bdv' size='340' side='right'caption='[[7bdv]], [[Resolution|resolution]] 2.02&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7bdv]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfobacillus_thermosulfidooxidans Sulfobacillus thermosulfidooxidans] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BDV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BDV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.02&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bdv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bdv OCA], [https://pdbe.org/7bdv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bdv RCSB], [https://www.ebi.ac.uk/pdbsum/7bdv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bdv ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cells and organisms have a wide range of mechanisms to defend against infection by viruses and other mobile genetic elements (MGE). Type III CRISPR systems detect foreign RNA and typically generate cyclic oligoadenylate (cOA) second messengers that bind to ancillary proteins with CARF (CRISPR associated Rossman fold) domains. This results in the activation of fused effector domains for antiviral defence. The best characterised CARF family effectors are the Csm6/Csx1 ribonucleases and DNA nickase Can1. Here we investigate a widely distributed CARF family effector with a nuclease domain, which we name Can2 (CRISPR ancillary nuclease 2). Can2 is activated by cyclic tetra-adenylate (cA4) and displays both DNase and RNase activity, providing effective immunity against plasmid transformation and bacteriophage infection in Escherichia coli. The structure of Can2 in complex with cA4 suggests a mechanism for the cA4-mediated activation of the enzyme, whereby an active site cleft is exposed on binding the activator. These findings extend our understanding of type III CRISPR cOA signalling and effector function.

The CRISPR ancillary effector Can2 is a dual-specificity nuclease potentiating type III CRISPR defence.,Zhu W, McQuarrie S, Gruschow S, McMahon SA, Graham S, Gloster TM, White MF Nucleic Acids Res. 2021 Mar 18;49(5):2777-2789. doi: 10.1093/nar/gkab073. PMID:33590098<ref>PMID:33590098</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7bdv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Sulfobacillus thermosulfidooxidans]]
[[Category: Synthetic construct]]
[[Category: Gloster TM]]
[[Category: Graham S]]
[[Category: Gruschow S]]
[[Category: McMahon SA]]
[[Category: McQuarrie S]]
[[Category: White MF]]
[[Category: Zhu W]]