View source for 7bfq


==Structure of the Integrator cleavage module with extended INTS4 and rigid body docked INTS9/11 CTD==
<StructureSection load='7bfq' size='340' side='right'caption='[[7bfq]], [[Resolution|resolution]] 4.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7bfq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BFQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BFQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.15&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bfq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bfq OCA], [https://pdbe.org/7bfq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bfq RCSB], [https://www.ebi.ac.uk/pdbsum/7bfq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bfq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/INT9_HUMAN INT9_HUMAN] Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
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== Publication Abstract from PubMed ==
The Integrator is a specialized 3' end-processing complex involved in cleavage and transcription termination of a subset of nascent RNA polymerase II transcripts, including small nuclear RNAs (snRNAs). We provide evidence of the modular nature of the Integrator complex by biochemically characterizing its two subcomplexes, INTS5/8 and INTS10/13/14. Using cryoelectron microscopy (cryo-EM), we determined a 3.5-A-resolution structure of the INTS4/9/11 ternary complex, which constitutes Integrator's catalytic core. Our structure reveals the spatial organization of the catalytic nuclease INTS11, bound to its catalytically impaired homolog INTS9 via several interdependent interfaces. INTS4, a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. In this assembly, all three proteins form a composite electropositive groove, suggesting a putative RNA binding path within the complex. Comparison with other 3' end-processing machineries points to distinct features and a unique architecture of the Integrator's catalytic module.

Structure of the catalytic core of the Integrator complex.,Pfleiderer MM, Galej WP Mol Cell. 2021 Mar 18;81(6):1246-1259.e8. doi: 10.1016/j.molcel.2021.01.005. Epub , 2021 Feb 5. PMID:33548203<ref>PMID:33548203</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 7bfq" style="background-color:#fffaf0;"></div>

==See Also==
*[[Integrator complex 3D structures|Integrator complex 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Galej WP]]
[[Category: Pfleiderer MM]]