View source for 7btp


==EcoR124I-Ocr in Restriction-Alleviation State==
<StructureSection load='7btp' size='340' side='right'caption='[[7btp]], [[Resolution|resolution]] 4.01&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7btp]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BTP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.01&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7btp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7btp OCA], [https://pdbe.org/7btp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7btp RCSB], [https://www.ebi.ac.uk/pdbsum/7btp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7btp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/T1S1_ECOLX T1S1_ECOLX] The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The EcoR124/3i enzyme recognizes 5'-GAAN(6)RTCG-3'.
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia phage T7]]
[[Category: Large Structures]]
[[Category: Gao P]]
[[Category: Gao Y]]