View source for 7bw5


==a new lasso peptide koreensin==
<StructureSection load='7bw5' size='340' side='right'caption='[[7bw5]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7bw5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingomonas_koreensis Sphingomonas koreensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BW5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BW5 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bw5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bw5 OCA], [https://pdbe.org/7bw5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bw5 RCSB], [https://www.ebi.ac.uk/pdbsum/7bw5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bw5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2M8WFL4_9SPHN A0A2M8WFL4_9SPHN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lasso peptides are a class of ribosomally biosynthesized and posttranslationally modified peptides with a knot structure as a common motif. Based on a genome search, a new biosynthetic gene cluster of lasso peptide was found in the genome of the proteobacterium Sphingomonas koreensis. Interestingly, the amino acid sequence of the precursor peptide gene includes two cell adhesion motif sequences (KGD and DGR). Heterologous production of the new lasso peptide was performed using the cryptic biosynthetic gene cluster of S. koreensis. As a result, a new lasso peptide named koreensin was produced by the gene expression system in the host strain Sphingomonas subterranea. The structure of koreensin was determined by NMR and ESI-MS analysis. The three-dimensional structure of koreensin was obtained based on an NOE experiment and the coupling constants. A variant peptide (koreensin-RGD), which had RGD instead of KGD, was produced by heterologous production with site-directed mutagenesis experiment. Koreensin and koreensin-RGD did not show cell adhesion inhibitory activity, although the molecules possessed cell adhesion motifs. The possible presence of a salt bridge between the motifs in koreensin was indicated, and it may prevent the cell adhesion motif from functioning.

Heterologous production of new lasso peptide koreensin based on genome mining.,Fuwa H, Hemmi H, Kaweewan I, Kozaki I, Honda H, Kodani S J Antibiot (Tokyo). 2020 Aug 27. pii: 10.1038/s41429-020-00363-5. doi:, 10.1038/s41429-020-00363-5. PMID:32855516<ref>PMID:32855516</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7bw5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Sphingomonas koreensis]]
[[Category: Hemmi H]]
[[Category: Kodani S]]