View source for 7bwx


==Crystal structure of ice-binding protein from an Antarctic ascomycete, Antarctomyces psychrotrophicus.==
<StructureSection load='7bwx' size='340' side='right'caption='[[7bwx]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7bwx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Antarctomyces_psychrotrophicus Antarctomyces psychrotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BWX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.904&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bwx OCA], [https://pdbe.org/7bwx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bwx RCSB], [https://www.ebi.ac.uk/pdbsum/7bwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bwx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2Z6DSM4_ANTPS A0A2Z6DSM4_ANTPS] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Many microbes that survive in cold environments are known to secrete ice-binding proteins (IBPs). The structure-function relationship of these proteins remains unclear. A microbial IBP denoted AnpIBP was recently isolated from a cold-adapted fungus, Antarctomyces psychrotrophicus. The present study identified an orbital illumination (prism ring) on a globular single ice crystal when soaked in a solution of fluorescent AnpIBP, suggesting that AnpIBP binds to specific water molecules located in the ice prism planes. In order to examine this unique ice-binding mechanism, we carried out X-ray structural analysis and mutational experiments. It appeared that AnpIBP is made of 6-ladder beta-helices with a triangular cross section that accompanies an "ice-like" water network on the ice-binding site. The network, however, does not exist in a defective mutant. AnpIBP has a row of four unique hollows on the IBS, where the distance between the hollows (14.7 A) is complementary to the oxygen atom spacing of the prism ring. These results suggest the structure of AnpIBP is fine-tuned to merge with the ice-water interface of an ice crystal through its polygonal water network and is then bound to a specific set of water molecules constructing the prism ring to effectively halt the growth of ice.

An Ice-Binding Protein from an Antarctic Ascomycete Is Fine-Tuned to Bind to Specific Water Molecules Located in the Ice Prism Planes.,Yamauchi A, Arai T, Kondo H, Sasaki YC, Tsuda S Biomolecules. 2020 May 13;10(5). pii: biom10050759. doi: 10.3390/biom10050759. PMID:32414092<ref>PMID:32414092</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7bwx" style="background-color:#fffaf0;"></div>

==See Also==
*[[Antifreeze protein 3D structures|Antifreeze protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Antarctomyces psychrotrophicus]]
[[Category: Large Structures]]
[[Category: Arai T]]
[[Category: Kondo H]]
[[Category: Tsuda S]]
[[Category: Yamauchi A]]