View source for 7bze


==Structure of Bacillus subtilis HxlR, K13A mutant==
<StructureSection load='7bze' size='340' side='right'caption='[[7bze]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7bze]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BZE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.658&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bze OCA], [https://pdbe.org/7bze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bze RCSB], [https://www.ebi.ac.uk/pdbsum/7bze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bze ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HXLR_BACSU HXLR_BACSU] Positive regulator of hxlAB expression.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Formaldehyde (FA) has long been considered as a toxin and carcinogen due to its damaging effects to biological macromolecules, but its beneficial roles have been increasingly appreciated lately. Real-time monitoring of this reactive molecule in living systems is highly desired in order to decipher its physiological and/or pathological functions, but a genetically encoded FA sensor is currently lacking. We herein adopt a structure-based study of the underlying mechanism of the FA-responsive transcription factor HxlR from Bacillus subtilis, which shows that HxlR recognizes FA through an intra-helical cysteine-lysine crosslinking reaction at its N-terminal helix alpha1, leading to conformational change and transcriptional activation. By leveraging this FA-induced intra-helical crosslinking and gain-of-function reorganization, we develop the genetically encoded, reaction-based FA sensor-FAsor, allowing spatial-temporal visualization of FA in mammalian cells and mouse brain tissues.

Genetically encoded formaldehyde sensors inspired by a protein intra-helical crosslinking reaction.,Zhu R, Zhang G, Jing M, Han Y, Li J, Zhao J, Li Y, Chen PR Nat Commun. 2021 Jan 25;12(1):581. doi: 10.1038/s41467-020-20754-4. PMID:33495458<ref>PMID:33495458</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7bze" style="background-color:#fffaf0;"></div>

==See Also==
*[[Tetracycline repressor protein 3D structures|Tetracycline repressor protein 3D structures]]
*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[Category: Large Structures]]
[[Category: Chen PR]]
[[Category: Zhu R]]