View source for 7c08


==Crystal structure of S34Y mutant of yeast U2AF1 complex bound to 3' splice site RNA, 5'-UAGGU.==
<StructureSection load='7c08' size='340' side='right'caption='[[7c08]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7c08]] is a 27 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C08 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C08 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.35&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c08 OCA], [https://pdbe.org/7c08 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c08 RCSB], [https://www.ebi.ac.uk/pdbsum/7c08 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c08 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/U2AF1_SCHPO U2AF1_SCHPO] Necessary for the splicing of pre-mRNA. The SF1-U2AF59-U2AF23 complex has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.<ref>PMID:12374752</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The accurate exclusion of introns by RNA splicing is critical for the production of mature mRNA. U2AF1 binds specifically to the 3 splice site, which includes an essential AG dinucleotide. Even a single amino acid mutation of U2AF1 can cause serious disease such as certain cancers or myelodysplastic syndromes. Here, we describe the first crystal structures of wild-type and pathogenic mutant U2AF1 complexed with target RNA, revealing the mechanism of 3 splice site selection, and how aberrant splicing results from clinically important mutations. Unexpected features of this mechanism may assist the future development of new treatments against diseases caused by splicing errors.

Elucidation of the aberrant 3' splice site selection by cancer-associated mutations on the U2AF1.,Yoshida H, Park SY, Sakashita G, Nariai Y, Kuwasako K, Muto Y, Urano T, Obayashi E Nat Commun. 2020 Sep 21;11(1):4744. doi: 10.1038/s41467-020-18559-6. PMID:32958768<ref>PMID:32958768</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7c08" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Schizosaccharomyces pombe 972h-]]
[[Category: Synthetic construct]]
[[Category: Obayashi E]]
[[Category: Park SY]]
[[Category: Urano T]]
[[Category: Yoshida H]]