View source for 7cjr


==Crystal structure of a periplasmic sensor domain of histidine kinase VbrK==
<StructureSection load='7cjr' size='340' side='right'caption='[[7cjr]], [[Resolution|resolution]] 2.28&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7cjr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"oceanomonas_parahaemolytica"_(fujino_et_al._1951)_miyamoto_et_al._1961 "oceanomonas parahaemolytica" (fujino et al. 1951) miyamoto et al. 1961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CJR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7CJR FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CGJ74_25135, WR32_19055 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=670 "Oceanomonas parahaemolytica" (Fujino et al. 1951) Miyamoto et al. 1961])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7cjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cjr OCA], [http://pdbe.org/7cjr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7cjr RCSB], [http://www.ebi.ac.uk/pdbsum/7cjr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7cjr ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacterial two-component regulatory systems (TCS) play important roles in sensing environmental stimuli and responding to them by regulating gene expression. VbrK/VbrR, a TCS in Vibrio parahaemolyticus, confers resistance to beta-lactam antibiotics through activating a beta-lactamase gene. Its periplasmic sensor domain was previously suggested to detect beta-lactam antibiotics by direct binding. Here, we report a crystal structure of the periplasmic sensing domain of VbrK (VbrK(SD)) using sulfur-based single-wavelength anomalous diffraction (S-SAD) phasing. Contrary to most bacterial sensor domains which form dimers, we show that VbrK(SD) is a monomer using size exclusion chromatography coupled with multi-angle light scattering. This observation is also supported by molecular dynamics simulations. To quantify the binding affinity of beta-lactam antibiotics to VbrK(SD), we performed isothermal titration calorimetry and other biophysical analyses. Unexpectedly, VbrK(SD) did not show any significant binding to beta-lactam antibiotics. Therefore, we propose that the detection of beta-lactam antibiotics by VbrK is likely to be indirect via an as yet unidentified mechanism.

Crystal structure of the periplasmic sensor domain of histidine kinase VbrK suggests indirect sensing of beta-lactam antibiotics.,Goh BC, Chua YK, Qian X, Lin J, Savko M, Dedon PC, Lescar J J Struct Biol. 2020 Sep 2;212(2):107610. doi: 10.1016/j.jsb.2020.107610. PMID:32890780<ref>PMID:32890780</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7cjr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Histidine kinase]]
[[Category: Large Structures]]
[[Category: Chua, Y K]]
[[Category: Goh, B C]]
[[Category: Lescar, J]]
[[Category: Qian, X]]
[[Category: Savko, M]]
[[Category: Sensor]]
[[Category: Signaling protein]]
[[Category: Tetratricopeptide repeat]]
[[Category: Transferase]]