View source for 7cli


==Structure of NF-kB p52 homodimer bound to P-Selectin kB DNA fragment==
<StructureSection load='7cli' size='340' side='right'caption='[[7cli]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7cli]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CLI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cli OCA], [https://pdbe.org/7cli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cli RCSB], [https://www.ebi.ac.uk/pdbsum/7cli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cli ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The mammalian NF-kappaB p52:p52 homodimer together with its cofactor Bcl3 activates transcription of kappaB sites with a central G/C base pair (bp), while it is inactive toward kappaB sites with a central A/T bp. To understand the molecular basis for this unique property of p52, we have determined the crystal structures of recombinant human p52 protein in complex with a P-selectin(PSel)-kappaB DNA (5'-GGGGTGACCCC-3') (central bp is underlined) and variants changing the central bp to A/T or swapping the flanking bp. The structures reveal a nearly two-fold widened minor groove in the central region of the DNA as compared to all other currently available NF-kappaB-DNA complex structures, which have a central A/T bp. Microsecond molecular dynamics (MD) simulations of free DNAs and p52 bound complexes reveal that free DNAs exhibit distinct preferred conformations, and p52:p52 homodimer induces the least amount of DNA conformational changes when bound to the more transcriptionally active natural G/C-centric PSel-kappaB, but adopts closed conformation when bound to the mutant A/T and swap DNAs due to their narrowed minor grooves. Our binding assays further demonstrate that the fast kinetics favored by entropy is correlated with higher transcriptional activity. Overall, our studies have revealed a novel conformation for kappaB DNA in complex with NF-kappaB and pinpoint the importance of binding kinetics, dictated by DNA conformational and dynamic states, in controlling transcriptional activation for NF-kappaB.

Structures of NF-kappaB p52 homodimer-DNA complexes rationalize binding mechanisms and transcription activation.,Pan W, Meshcheryakov VA, Li T, Wang Y, Ghosh G, Wang VY Elife. 2023 Feb 13;12:e86258. doi: 10.7554/eLife.86258. PMID:36779700<ref>PMID:36779700</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7cli" style="background-color:#fffaf0;"></div>

==See Also==
*[[NF-kB|NF-kB]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Meshcheryakov VA]]
[[Category: Wang VY-F]]