View source for 7cls


==Solution structure of an intramolecular G-quadruplex containing a duplex bulge==
<StructureSection load='7cls' size='340' side='right'caption='[[7cls]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7cls]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CLS FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cls OCA], [https://pdbe.org/7cls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cls RCSB], [https://www.ebi.ac.uk/pdbsum/7cls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cls ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Beyond the consensus definition of G-quadruplex-forming motifs with tracts of continuous guanines, G-quadruplexes harboring bulges in the G-tetrad core are prevalent in the human genome. Here, we study the incorporation of a duplex hairpin within a bulge of a G-quadruplex. The NMR solution structure of a G-quadruplex containing a duplex bulge was resolved, revealing the structural details of the junction between the duplex bulge and the G-quadruplex. Unexpectedly, instead of an orthogonal connection the duplex stem was observed to stack below the G-quadruplex forming a unique quadruplex-duplex junction. Breaking up of the immediate base pair step at the junction, coupled with a narrowing of the duplex groove within the context of the bulge, led to a progressive transition between the quadruplex and duplex segments. This study revealed that a duplex bulge can be formed at various positions of a G-quadruplex scaffold. In contrast to a non-structured bulge, the stability of a G-quadruplex slightly increases with an increase in the duplex bulge size. A G-quadruplex structure containing a duplex bulge of up to 33 nt in size was shown to form, which was much larger than the previously reported 7-nt bulge. With G-quadruplexes containing duplex bulges representing new structural motifs with potential biological significance, our findings would broaden the definition of potential G-quadruplex-forming sequences.

Duplex formation in a G-quadruplex bulge.,Ngoc Nguyen TQ, Lim KW, Phan AT Nucleic Acids Res. 2020 Sep 22. pii: 5909921. doi: 10.1093/nar/gkaa738. PMID:32960213<ref>PMID:32960213</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Lim KW]]
[[Category: Nguyen TQN]]
[[Category: Phan AT]]