View source for 7d34


==AtClpS1-peptide complex==
<StructureSection load='7d34' size='340' side='right'caption='[[7d34]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7d34]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D34 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D34 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.007&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=ALA:ALANINE'>ALA</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d34 OCA], [https://pdbe.org/7d34 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d34 RCSB], [https://www.ebi.ac.uk/pdbsum/7d34 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d34 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CLPS1_ARATH CLPS1_ARATH] Small adapter protein that modulate the activity of CLPC (By similarity). Involved in plastid biogenesis in particular when chloroplast protein synthesis capacity is a limiting factor (PubMed:23898032). Probably involved in substrate selection for plastid Clp protease system (PubMed:23898032). Recruitment to ClpC chaperones is facilitated by CLPF thus forming a binary adapter for selective substrate recognition and delivery to plastid Clp protease system (CLPC) (PubMed:26419670).[UniProtKB:P0A8Q6]<ref>PMID:23898032</ref> <ref>PMID:26419670</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The N-degron pathway determines the half-life of proteins in both prokaryotes and eukaryotes by precisely recognizing the N-terminal residue (N-degron) of substrates. ClpS proteins from bacteria bind to substrates containing hydrophobic N-degrons (Leu, Phe, Tyr, and Trp) and deliver them to the caseinolytic protease system ClpAP. This mechanism is preserved in organelles such as mitochondria and chloroplasts. Bacterial ClpS adaptors bind preferentially to Leu and Phe N-degrons; however, ClpS1 from Arabidopsis thaliana (AtClpS1) shows a difference in that it binds strongly to Phe and Trp N-degrons and only weakly to Leu. This difference in behavior cannot be explained without structural information due to the high sequence homology between bacterial and plant ClpS proteins. Here, we report the structure of AtClpS1 at 2.0 A resolution in the presence of a bound N-degron. The key determinants for alpha-amino group recognition are conserved among all ClpS proteins, but the alpha3-helix of eukaryotic AtClpS1 is significantly shortened, and consequently, a loop forming a pocket for the N-degron is moved slightly outward to enlarge the pocket. In addition, amino acid replacement from Val to Ala causes a reduction in hydrophobic interactions with Leu N-degron. A combination of the fine-tuned hydrophobic residues in the pocket and the basic gatekeeper at the entrance of the pocket controls the N-degron selectivity of the plant ClpS protein.

Structural basis for the N-degron specificity of ClpS1 from Arabidopsis thaliana.,Kim L, Heo J, Kwon DH, Shin JS, Jang SH, Park ZY, Song HK Protein Sci. 2021 Mar;30(3):700-708. doi: 10.1002/pro.4018. Epub 2020 Dec 30. PMID:33368743<ref>PMID:33368743</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7d34" style="background-color:#fffaf0;"></div>

==See Also==
*[[ATP-dependent Clp protease adaptor protein 3D structures|ATP-dependent Clp protease adaptor protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Heo J]]
[[Category: Kim L]]
[[Category: Kwon DH]]
[[Category: Song HK]]