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==Crystal Structures of Anthocyanin 5,3'-aromatic acyltransferase from Gentiana triflora==
<StructureSection load='7dev' size='340' side='right'caption='[[7dev]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7dev]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gentiana_triflora Gentiana triflora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DEV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DEV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dev OCA], [https://pdbe.org/7dev PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dev RCSB], [https://www.ebi.ac.uk/pdbsum/7dev PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dev ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ANTA_GENTR ANTA_GENTR] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The acylation of anthocyanins contributes to their structural diversity. Aromatic acylation is responsible for the blue color of anthocyanins and certain flowers. Aromatic acyltransferase from Gentiana triflora Pall. (Gentianaceae) (Gt5,3'AT) catalyzes the acylation of glucosyl moieties at the 5 and 3' positions of anthocyanins. Anthocyanin acyltransferase transfers an acyl group to a single position, such that Gt5,3'AT possesses a unique enzymatic activity. Structural investigation of this aromatic acyl group transfer is fundamental to understand the molecular mechanism of the acylation of double positions. In this study, structural analyses of Gt5,3'AT were conducted to identify the underlying mechanism. The crystal structure indicated that Gt5,3'AT shares structural similarities with other BAHD family enzymes, consisting of N and C terminal lobes. Structural comparison revealed that acyl group preference (aromatic or aliphatic) for the enzymes was determined by four amino acid positions, which are well conserved in aromatic and aliphatic CoA-binding acyltransferases. Although a complex structure with anthocyanins was not obtained, the binding of delphinidin 3,5,3'-triglucoside to Gt5,3'AT was investigated by evaluating the molecular dynamics. The simulation indicated that acyl transfer by Gt5,3'AT preferentially occurs at the 5-position rather than at the 3'-position, with interacting amino acids that are mainly located in the C-terminal lobe. Subsequent assays of chimeric enzymes (exchange of the N-terminal lobe and the C-terminal lobe between Gt5,3'AT and lisianthus anthocyanin 5AT) demonstrated that acyl transfer selectivity may be caused by the C-terminal lobe.

Anthocyanin 5,3'-aromatic acyltransferase from Gentiana triflora, a structural insight into biosynthesis of a blue anthocyanin.,Murayama K, Kato-Murayama M, Sato T, Hosaka T, Ishiguro K, Mizuno T, Kitao K, Honma T, Yokoyama S, Tanaka Y, Shirouzu M Phytochemistry. 2021 Jun;186:112727. doi: 10.1016/j.phytochem.2021.112727. Epub, 2021 Mar 18. PMID:33743393<ref>PMID:33743393</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7dev" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Gentiana triflora]]
[[Category: Large Structures]]
[[Category: Kato-Murayama M]]
[[Category: Murayama K]]
[[Category: Shirouzu M]]