View source for 7dqk


==A nicotine MATE transporter, Nicotiana tabacum MATE2 (NtMATE2)==
<StructureSection load='7dqk' size='340' side='right'caption='[[7dqk]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7dqk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DQK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dqk OCA], [https://pdbe.org/7dqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dqk RCSB], [https://www.ebi.ac.uk/pdbsum/7dqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dqk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A3KDM5_TOBAC A3KDM5_TOBAC] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A transporter of the multidrug and toxic compound extrusion (MATE) family, Nicotiana tabacum MATE2 (NtMATE2) is located in the vacuole membrane of the tobacco plant root and is involved in the transportation of nicotine, a secondary or specialized metabolic compound in Solanaceae. Here, we report the crystal structures of NtMATE2 in its outward-facing forms. The overall structure has a bilobate V-shape with pseudo-symmetrical assembly of the N- and C-lobes. In one crystal structure, the C-lobe cavity of NtMATE2 interacts with an unidentified molecule that may partially mimic a substrate. In addition, NtMATE2-specific conformational transitions imply that an unprecedented movement of the transmembrane alpha-helix 7 (TM7) is related to the release of the substrate into the vacuolar lumen.

Crystal structures of a nicotine MATE transporter provide insight into its mechanism of substrate transport.,Tanaka Y, Iwaki S, Sasaki A, Tsukazaki T FEBS Lett. 2021 May 29. doi: 10.1002/1873-3468.14136. PMID:34050946<ref>PMID:34050946</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7dqk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Nicotiana tabacum]]
[[Category: Iwaki S]]
[[Category: Sasaki A]]
[[Category: Tanaka Y]]
[[Category: Tsukazaki T]]