View source for 7drn


==Structure of ATP-grasp ligase PsnB complexed with precursor peptide PsnA2 and AMPPNP==
<StructureSection load='7drn' size='340' side='right'caption='[[7drn]], [[Resolution|resolution]] 3.56&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7drn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Plesiocystis_pacifica_SIR-1 Plesiocystis pacifica SIR-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DRN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DRN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.56&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7drn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7drn OCA], [https://pdbe.org/7drn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7drn RCSB], [https://www.ebi.ac.uk/pdbsum/7drn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7drn ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A6G4D7_9DELT A6G4D7_9DELT] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Graspetides, also known as omega-ester-containing peptides (OEPs), are a family of ribosomally synthesized and post-translationally modified peptides (RiPPs) bearing side chain-to-side chain macrolactone or macrolactam linkages. Here, we present the molecular details of precursor peptide recognition by the macrocyclase enzyme PsnB in the biosynthesis of plesiocin, a group 2 graspetide. Biochemical analysis revealed that, in contrast to other RiPPs, the core region of the plesiocin precursor peptide noticeably enhanced the enzyme-precursor interaction via the conserved glutamate residues. We obtained four crystal structures of symmetric or asymmetric PsnB dimers, including those with a bound core peptide and a nucleotide, and suggest that the highly conserved Arg213 at the enzyme active site specifically recognizes a ring-forming acidic residue before phosphorylation. Collectively, this study provides insights into the mechanism underlying substrate recognition in graspetide biosynthesis and lays a foundation for engineering new variants.

Molecular mechanism underlying substrate recognition of the peptide macrocyclase PsnB.,Song I, Kim Y, Yu J, Go SY, Lee HG, Song WJ, Kim S Nat Chem Biol. 2021 Nov;17(11):1123-1131. doi: 10.1038/s41589-021-00855-x. Epub , 2021 Sep 2. PMID:34475564<ref>PMID:34475564</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7drn" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Plesiocystis pacifica SIR-1]]
[[Category: Kim S]]
[[Category: Song I]]
[[Category: Song W]]
[[Category: Yu J]]