View source for 7dve


==Crystal structure of FAD-dependent C-glycoside oxidase==
<StructureSection load='7dve' size='340' side='right'caption='[[7dve]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7dve]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Microbacterium_trichothecenolyticum Microbacterium trichothecenolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DVE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DVE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dve FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dve OCA], [https://pdbe.org/7dve PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dve RCSB], [https://www.ebi.ac.uk/pdbsum/7dve PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dve ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0M2HFA3_9MICO A0A0M2HFA3_9MICO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
C-glycosides have a unique structure, in which an anomeric carbon of a sugar is directly bonded to the carbon of an aglycone skeleton. One of the natural C-glycosides, carminic acid, is utilized by the food, cosmetic, and pharmaceutical industries, for a total of more than 200 tons/y worldwide. However, a metabolic pathway of carminic acid has never been identified. In this study, we isolated the previously unknown carminic acid-catabolizing microorganism and discovered a flavoenzyme "C-glycoside 3-oxidase" named CarA that catalyzes oxidation of the sugar moiety of carminic acid. A Basic Local Alignment Search Tool (BLAST) search demonstrated that CarA homologs were distributed in soil microorganisms but not intestinal ones. In addition to CarA, two CarA homologs were cloned and heterologously expressed, and their biochemical properties were determined. Furthermore, a crystal structure of one homolog was determined. Together with the biochemical analysis, the crystal structure and a mutagenesis analysis of CarA revealed the mechanisms underlying their substrate specificity and catalytic reaction. Our study suggests that CarA and its homologs play a crucial role in the metabolism of C-glycosides in nature.

FAD-dependent C-glycoside-metabolizing enzymes in microorganisms: Screening, characterization, and crystal structure analysis.,Kumano T, Hori S, Watanabe S, Terashita Y, Yu HY, Hashimoto Y, Senda T, Senda M, Kobayashi M Proc Natl Acad Sci U S A. 2021 Oct 5;118(40). pii: 2106580118. doi:, 10.1073/pnas.2106580118. PMID:34583991<ref>PMID:34583991</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7dve" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Microbacterium trichothecenolyticum]]
[[Category: Kobayashi M]]
[[Category: Kumano T]]
[[Category: Senda M]]
[[Category: Senda T]]
[[Category: Watanabe S]]