View source for 7dwb


==Human Pannexin1 model==
<StructureSection load='7dwb' size='340' side='right'caption='[[7dwb]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DWB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DWB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.15&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dwb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dwb OCA], [https://pdbe.org/7dwb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dwb RCSB], [https://www.ebi.ac.uk/pdbsum/7dwb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dwb ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Pannexin1 (PANX1) is a large-pore ATP efflux channel with a broad distribution, which allows the exchange of molecules and ions smaller than 1 kDa between the cytoplasm and extracellular space. In this study, we show that in human macrophages PANX1 expression is upregulated by diverse stimuli that promote pyroptosis, which is reminiscent of the previously reported lipopolysaccharide-induced upregulation of PANX1 during inflammasome activation. To further elucidate the function of PANX1, we propose the full-length human Pannexin1 (hPANX1) model through cryo-electron microscopy (cryo-EM) and molecular dynamics (MD) simulation studies, establishing hPANX1 as a homo-heptamer and revealing that both the N-termini and C-termini protrude deeply into the channel pore funnel. MD simulations also elucidate key energetic features governing the channel that lay a foundation to understand the channel gating mechanism. Structural analyses, functional characterizations, and computational studies support the current hPANX1-MD model, suggesting the potential role of hPANX1 in pyroptosis during immune responses.

Structure of the full-length human Pannexin1 channel and insights into its role in pyroptosis.,Zhang S, Yuan B, Lam JH, Zhou J, Zhou X, Ramos-Mandujano G, Tian X, Liu Y, Han R, Li Y, Gao X, Li M, Yang M Cell Discov. 2021 May 4;7(1):30. doi: 10.1038/s41421-021-00259-0. PMID:33947837<ref>PMID:33947837</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7dwb" style="background-color:#fffaf0;"></div>

==See Also==
*[[Pannexin|Pannexin]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Yang MJ]]
[[Category: Zhang SS]]