View source for 7dws


==The structure of T4 Lysozyme I3C/C54T/R125C/E128C complex with Zinc ions==
<StructureSection load='7dws' size='340' side='right'caption='[[7dws]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DWS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dws OCA], [https://pdbe.org/7dws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dws RCSB], [https://www.ebi.ac.uk/pdbsum/7dws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dws ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
The development of recombinant protein cross-linked injectable hydrogels with good mechanical strength and effective drug loading capacity for bone regeneration is extremely attractive and rarely reported. Here, we report the fabrication of a smart hydrogel delivery system by incorporating a rationally designed T4 lysozyme mutant (T4M) to mediate the localized delivery and synergistic release of Mg(2+) and Zn(2+) for bone repair. Apart from its intrinsic antibacterial properties, T4M bears abundant free amine groups on its surface to function as effective covalent crosslinkers to strengthen the hydrogel network as well as exhibits specific binding affinity to multivalent cations such as Zn(2+). Moreover, the integrin receptor-binding Arg-Gly-Asp (RGD) sequence was introduced onto the C-terminus of T4 lysozyme to improve its cellular affinity and further facilitate rapid tissue regeneration. The final composite hydrogel displays excellent injectability, improved mechanical properties, antibacterial activity, and unique bioactivities. The effective loading of Mg(2+)/Zn(2+) in the hydrogels could mediate the sequential and sustained release of Mg(2+) and Zn(2+), thereby resulting in synergistic enhancement on bone regeneration through modulation of the MAPK signaling pathway. We believe that the strategy proposed in this paper opens up a new route for developing protein cross-linked smart delivery systems for tissue regeneration.

Rationally designed protein cross-linked hydrogel for bone regeneration via synergistic release of magnesium and zinc ions.,Chen X, Tan B, Wang S, Tang R, Bao Z, Chen G, Chen S, Tang W, Wang Z, Long C, Lu WW, Yang D, Bian L, Peng S Biomaterials. 2021 May 12;274:120895. doi: 10.1016/j.biomaterials.2021.120895. PMID:34020269<ref>PMID:34020269</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 7dws" style="background-color:#fffaf0;"></div>

==See Also==
*[[Lysin 3D structures|Lysin 3D structures]]
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Chen S]]
[[Category: Chen X]]