View source for 7ehk


==Crystal structure of C107S mutant of FfIBP==
<StructureSection load='7ehk' size='340' side='right'caption='[[7ehk]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ehk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Flavobacterium_frigoris_PS1 Flavobacterium frigoris PS1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EHK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ehk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ehk OCA], [https://pdbe.org/7ehk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ehk RCSB], [https://www.ebi.ac.uk/pdbsum/7ehk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ehk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IBP_FLAFP IBP_FLAFP] Has antifreeze activity for survival in a subzero environment. Binds to the surface of ice crystals and inhibits their growth. Has high thermal hysteresis (TH) activity, which is the ability to lower the freezing point of an aqueous solution below its melting point, and thus the freezing of the cell fluid can be prevented protecting the organism from ice damage (PubMed:22750870, PubMed:24699650, PubMed:27737617). The TH activity of this protein is 2.2 degrees Celsius at 5 uM and 2.5 degrees Celsius at 50 uM (PubMed:24699650).<ref>PMID:22750870</ref> <ref>PMID:24699650</ref> <ref>PMID:27737617</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ice-binding proteins (IBPs) are well-characterized proteins responsible for the cold-adaptation mechanisms. Despite extensive structural and biological investigation of IBPs and antifreeze proteins, only a few studies have considered the relationship between protein stabilization and thermal hysteresis (TH) activity as well as the implication of hyperactivity. Here, we investigated the important role of the head capping region in stabilization and the hyper-TH activity of FfIBP using molecular dynamics simulation. Data comparison revealed that residues on the ice-binding site of the hyperactive FfIBP are immobilized, which could be correlated with TH activity. Further comparison analysis indicated the disulfide bond in the head region is mainly involved in protein stabilization and is crucial for hyper-TH activity. This finding could also be generalized to known hyperactive IBPs. Furthermore, in mimicking the physiological conditions, bacteria with membrane-anchored FfIBP formed brine pockets in a TH activity-dependent manner. Cells with a higher number of TH-active IBPs showed an increased number of brine pockets, which may be beneficial for short- and long-term survival in cold environments by reducing the salt concentration. The newly identified conditions for hyper-TH activity and their implications on bacterial survival provide insights into novel mechanistic aspects of cold adaptation in polar microorganisms.

Importance of rigidity of ice-binding protein (FfIBP) for hyperthermal hysteresis activity and microbial survival.,Hwang J, Kim B, Lee MJ, Kim EJ, Cho SM, Lee SG, Han SJ, Kim K, Lee JH, Do H Int J Biol Macromol. 2022 Feb 9;204:485-499. doi: 10.1016/j.ijbiomac.2022.02.032. PMID:35149098<ref>PMID:35149098</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ehk" style="background-color:#fffaf0;"></div>

==See Also==
*[[Antifreeze protein 3D structures|Antifreeze protein 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Flavobacterium frigoris PS1]]
[[Category: Large Structures]]
[[Category: Do H]]
[[Category: Lee JH]]