View source for 7ejo


==Structure of ERH-2 bound to TOST-1==
<StructureSection load='7ejo' size='340' side='right'caption='[[7ejo]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ejo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EJO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.191&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ejo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ejo OCA], [https://pdbe.org/7ejo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ejo RCSB], [https://www.ebi.ac.uk/pdbsum/7ejo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ejo ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ERH2_CAEEL ERH2_CAEEL] Required for chromosome segregation and cell division in early embryos (PubMed:31216475). Component of the pid-1 and tost-1 variants of the PETISCO complexes, which have roles in the biogenesis of a class of 21 nucleotide PIWI-interacting RNAs (piRNAs) that possess a uracil residue at the 5'-end (also called 21U-RNAs) and embryogenesis, respectively (PubMed:31147388, PubMed:31216475). Within the tost-1 variant of the PETISCO complex binds to splice leader SL1 RNA fragments to possibly play a role in their processing (PubMed:31147388). Promotes the biogenesis of 21U-RNAs (PubMed:31216475).<ref>PMID:31147388</ref> <ref>PMID:31216475</ref> <ref>PMID:31216475</ref> [https://www.uniprot.org/uniprot/TOST1_CAEEL TOST1_CAEEL] Component of the tost-1 variant of the PETISCO complex which plays an essential role in embryogenesis (PubMed:31147388, PubMed:31216475). Within the complex acts as an adapter which binds to the complex via erh-2 (PubMed:31147388). Does not seem to play a role in the biogenesis of a class of 21 nucleotide PIWI-interacting RNAs (piRNAs) that possess a uracil residue at the 5'-end (also called 21U-RNAs) (PubMed:31147388). May inhibit 21U-RNA accumulation (PubMed:31216475). Required for chromosome segregation and cell division in early embryos (PubMed:31216475).<ref>PMID:31147388</ref> <ref>PMID:31216475</ref> <ref>PMID:31216475</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
By incorporating two mutually exclusive factors, PID-1 and TOST-1, C. elegans PICS complex plays important roles in piRNA biogenesis, chromosome segregation and cell division. We firstly map the interaction network between PICS subunits, then uncover the mechanisms underlying the interactions between PICS subunits by solving several complex structures, including those of TOFU-6/PICS-1, ERH-2/PICS-1, and ERH-2/TOST-1. Our biochemical experiment also demonstrates that PICS exists as an octamer consisting of two copies of each subunit. Combining structural analyses with mutagenesis experiments, we identify interfacial residues of PICS subunits that are critical for maintaining intact PICS complex in vitro. Furthermore, using genetics, cell biology and imaging experiments, we find that those mutants impairing the in vitro interaction network within PICS, also lead to dysfunction of PICS in vivo, including mislocalization of PICS, and reduced levels of piRNAs or aberrant chromosome segregation and cell division. Therefore, our work provides structural insights into understanding the PICS-mediated piRNA biogenesis and cell division.

Molecular basis for PICS-mediated piRNA biogenesis and cell division.,Wang X, Zeng C, Liao S, Zhu Z, Zhang J, Tu X, Yao X, Feng X, Guang S, Xu C Nat Commun. 2021 Sep 22;12(1):5595. doi: 10.1038/s41467-021-25896-7. PMID:34552083<ref>PMID:34552083</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ejo" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Caenorhabditis elegans]]
[[Category: Large Structures]]
[[Category: Wang X]]
[[Category: Xu C]]