View source for 7eto


==C1 CVSC-binding penton vertex in the virion capsid of Human Cytomegalovirus==
<StructureSection load='7eto' size='340' side='right'caption='[[7eto]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7eto]] is a 26 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_betaherpesvirus_5 Human betaherpesvirus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ETO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ETO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eto OCA], [https://pdbe.org/7eto PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eto RCSB], [https://www.ebi.ac.uk/pdbsum/7eto PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eto ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q6RXF2_HCMV Q6RXF2_HCMV] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
How the human cytomegalovirus (HCMV) genome-the largest among human herpesviruses-is packaged, retained, and ejected remains unclear. We present the in situ structures of the symmetry-mismatched portal and the capsid vertex-specific components (CVSCs) of HCMV. The 5-fold symmetric 10-helix anchor-uncommon among known portals-contacts the portal-encircling DNA, which is presumed to squeeze the portal as the genome packaging proceeds. We surmise that the 10-helix anchor dampens this action to delay the portal reaching a "head-full" packaging state, thus facilitating the large genome to be packaged. The 6-fold symmetric turret, latched via a coiled coil to a helix from a major capsid protein, supports the portal to retain the packaged genome. CVSCs at the penton vertices-presumed to increase inner capsid pressure-display a low stoichiometry, which would aid genome retention. We also demonstrate that the portal and capsid undergo conformational changes to facilitate genome ejection after viral cell entry.

Structural basis for genome packaging, retention, and ejection in human cytomegalovirus.,Li Z, Pang J, Dong L, Yu X Nat Commun. 2021 Jul 27;12(1):4538. doi: 10.1038/s41467-021-24820-3. PMID:34315863<ref>PMID:34315863</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7eto" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Human betaherpesvirus 5]]
[[Category: Large Structures]]
[[Category: Li Z]]
[[Category: Yu X]]