View source for 7f2i


==Arabidopsis thaliana protease-associated domain of vacuolar-sorting receptor 1 in complex with cruciferin 1 C-terminal pentapeptide RVAAA (pH6.5)==
<StructureSection load='7f2i' size='340' side='right'caption='[[7f2i]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7f2i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F2I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F2I FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f2i OCA], [https://pdbe.org/7f2i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f2i RCSB], [https://www.ebi.ac.uk/pdbsum/7f2i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f2i ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/VSR1_ARATH VSR1_ARATH] Vacuolar-sorting receptor (VSR) involved in clathrin-coated vesicles sorting from Golgi apparatus to vacuoles. Required for the sorting of 12S globulin, 2S albumin and maybe other seed storage proteins to protein storage vacuoles (PSVs) in seeds. May also be implicated in targeting N-terminal propeptide containing proteins to lytic vacuoles.<ref>PMID:10871276</ref> <ref>PMID:14657332</ref> 
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== Publication Abstract from PubMed ==
In Arabidopsis, vacuolar sorting receptor isoform 1 (VSR1) sorts 12S globulins to the protein storage vacuoles during seed development. Vacuolar sorting is mediated by specific protein-protein interactions between VSR1 and the vacuolar sorting determinant located at the C terminus (ctVSD) on the cargo proteins. Here, we determined the crystal structure of the protease-associated domain of VSR1 (VSR1-PA) in complex with the C-terminal pentapeptide ((468)RVAAA(472)) of cruciferin 1, an isoform of 12S globulins. The (468)RVA(470) motif forms a parallel beta-sheet with the switch III residues ((127)TMD(129)) of VSR1-PA, and the (471)AA(472) motif docks to a cradle formed by the cargo-binding loop ((95)RGDCYF(100)), making a hydrophobic interaction with Tyr99. The C-terminal carboxyl group of the ctVSD is recognized by forming salt bridges with Arg95. The C-terminal sequences of cruciferin 1 and vicilin-like storage protein 22 were sufficient to redirect the secretory red fluorescent protein (spRFP) to the vacuoles in Arabidopsis protoplasts. Adding a proline residue to the C terminus of the ctVSD and R95M substitution of VSR1 disrupted receptor-cargo interactions in vitro and led to increased secretion of spRFP in Arabidopsis protoplasts. How VSR1-PA recognizes ctVSDs of other storage proteins was modeled. The last three residues of ctVSD prefer hydrophobic residues because they form a hydrophobic cluster with Tyr99 of VSR1-PA. Due to charge-charge interactions, conserved acidic residues, Asp129 and Glu132, around the cargo-binding site should prefer basic residues over acidic ones in the ctVSD. The structural insights gained may be useful in targeting recombinant proteins to the protein storage vacuoles in seeds.

Structural insights into how vacuolar sorting receptors recognize the sorting determinants of seed storage proteins.,Tsao HE, Lui SN, Lo AH, Chen S, Wong HY, Wong CK, Jiang L, Wong KB Proc Natl Acad Sci U S A. 2022 Jan 4;119(1):e2111281119. doi: , 10.1073/pnas.2111281119. PMID:34983843<ref>PMID:34983843</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 7f2i" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Lui SN]]
[[Category: Wong KB]]