View source for 7fbw


==Acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis==
<StructureSection load='7fbw' size='340' side='right'caption='[[7fbw]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7fbw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis_MB4 Caldanaerobacter subterraneus subsp. tengcongensis MB4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FBW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fbw OCA], [https://pdbe.org/7fbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fbw RCSB], [https://www.ebi.ac.uk/pdbsum/7fbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fbw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8RBF4_CALS4 Q8RBF4_CALS4] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The acetylxylan esterases (AXEs) classified into carbohydrate esterase family 4 (CE4) are metalloenzymes that catalyze the deacetylation of acetylated carbohydrates. AXE from Caldanaerobacter subterraneus subsp. tengcongensis (TTE0866), which belongs to CE4, is composed of three parts: a signal sequence (residues 1-22), an N-terminal region (NTR; residues 23-135) and a catalytic domain (residues 136-324). TTE0866 catalyzes the deacetylation of highly substituted cellulose acetate and is expected to be useful for industrial applications in the reuse of resources. In this study, the crystal structure of TTE0866 (residues 23-324) was successfully determined. The crystal diffracted to 1.9 A resolution and belonged to space group I212121. The catalytic domain (residues 136-321) exhibited a (beta/alpha)7-barrel topology. However, electron density was not observed for the NTR (residues 23-135). The crystal packing revealed the presence of an intermolecular space without observable electron density, indicating that the NTR occupies this space without a defined conformation or was truncated during the crystallization process. Although the active-site conformation of TTE0866 was found to be highly similar to those of other CE4 enzymes, the orientation of its Trp264 side chain near the active site was clearly distinct. The unique orientation of the Trp264 side chain formed a different-shaped cavity within TTE0866, which may contribute to its reactivity towards highly substituted cellulose acetate.

Crystal structure of acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis.,Sasamoto K, Himiyama T, Moriyoshi K, Ohmoto T, Uegaki K, Nishiya Y, Nakamura T Acta Crystallogr F Struct Biol Commun. 2021 Nov 1;77(Pt 11):399-406. doi:, 10.1107/S2053230X21009675. Epub 2021 Oct 19. PMID:34726178<ref>PMID:34726178</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7fbw" style="background-color:#fffaf0;"></div>

==See Also==
*[[Acetylxylan esterase 3D structures|Acetylxylan esterase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Caldanaerobacter subterraneus subsp. tengcongensis MB4]]
[[Category: Large Structures]]
[[Category: Himiyama T]]
[[Category: Moriyoshi K]]
[[Category: Nakamura T]]
[[Category: Nishiya Y]]
[[Category: Ohmoto T]]
[[Category: Sasamoto K]]
[[Category: Uegaki K]]