View source for 7fdf


==The E145S mutant of the regulatory domain of YeiE, a sulfite sensing LysR-type transcriptional regulator from Cronobacter sakazakii (sulfate-bound form)==
<StructureSection load='7fdf' size='340' side='right'caption='[[7fdf]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7fdf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cronobacter_sakazakii Cronobacter sakazakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FDF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FDF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fdf OCA], [https://pdbe.org/7fdf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fdf RCSB], [https://www.ebi.ac.uk/pdbsum/7fdf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fdf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/H9BVC9_CROSK H9BVC9_CROSK] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
SignificanceYeiE has been identified as a master virulence factor of Cronobacter sakazakii. In this study, we determined the crystal structures of the regulatory domain of YeiE in complex with its physiological ligand sulfite ion (SO3(2-)). The structure provides the basis for the molecular mechanisms for sulfite sensing and the ligand-dependent conformational changes of the regulatory domain. The genes under the control of YeiE in response to sulfite were investigated to reveal the functional roles of YeiE in the sulfite tolerance of the bacteria. We propose the molecular mechanism underlying the ability of gram-negative pathogens to defend against the innate immune response involving sulfite, thus providing a strategy to control the pathogenesis of bacteria.

Crystal structures of YeiE from Cronobacter sakazakii and the role of sulfite tolerance in gram-negative bacteria.,Hong S, Kim J, Cho E, Na S, Yoo YJ, Cho YH, Ryu S, Ha NC Proc Natl Acad Sci U S A. 2022 Mar 15;119(11):e2118002119. doi:, 10.1073/pnas.2118002119. Epub 2022 Mar 10. PMID:35271389<ref>PMID:35271389</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7fdf" style="background-color:#fffaf0;"></div>

==See Also==
*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Cronobacter sakazakii]]
[[Category: Large Structures]]
[[Category: Ha N-C]]
[[Category: Hong S]]