View source for 7fgg


==Cryo-EM Structure of Chikungunya Virus Nonstructural Protein 1 with m7GTP==
<StructureSection load='7fgg' size='340' side='right'caption='[[7fgg]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7fgg]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Chikungunya_virus_strain_S27-African_prototype Chikungunya virus strain S27-African prototype]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FGG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FGG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.19&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MGP:7-METHYL-GUANOSINE-5-TRIPHOSPHATE'>MGP</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fgg OCA], [https://pdbe.org/7fgg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fgg RCSB], [https://www.ebi.ac.uk/pdbsum/7fgg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fgg ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Many viruses encode RNA-modifying enzymes to edit the 5' end of viral RNA to mimic the cellular mRNA for effective protein translation, genome replication, and evasion of the host defense mechanisms. Alphavirus nsP1 synthesizes the 5' end Cap-0 structure of viral RNAs. However, the molecular basis of the capping process remains unclear. We determine high-resolution cryoelectron microscopy (cryo-EM) structures of Chikungunya virus nsP1 in complex with m7GTP/SAH, covalently attached m7GMP, and Cap-0 viral RNA. These structures reveal details of viral-RNA-capping reactions and uncover a sequence-specific virus RNA-recognition pattern that, in turn, regulates viral-RNA-capping efficiency to ensure optimal genome replication and subgenomic RNA transcription. This sequence-specific enzyme-RNA pairing is conserved across all alphaviruses.

Molecular basis of specific viral RNA recognition and 5'-end capping by the Chikungunya virus nsP1.,Zhang K, Law MCY, Nguyen TM, Tan YB, Wirawan M, Law YS, Jeong LS, Luo D Cell Rep. 2022 Jul 26;40(4):111133. doi: 10.1016/j.celrep.2022.111133. PMID:35905713<ref>PMID:35905713</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 7fgg" style="background-color:#fffaf0;"></div>

==See Also==
*[[Nonstructural protein 3D structures|Nonstructural protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Chikungunya virus strain S27-African prototype]]
[[Category: Large Structures]]
[[Category: Law MCY]]
[[Category: Law YS]]
[[Category: Luo DH]]
[[Category: Nguyen TM]]
[[Category: Tan YB]]
[[Category: Wirawan M]]
[[Category: Zhang K]]