View source for 7fiv


==Crystal structure of the complex formed by Wolbachia cytoplasmic incompatibility factors CidA and CidBND1-ND2 from wPip(Tunis)==
<StructureSection load='7fiv' size='340' side='right'caption='[[7fiv]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7fiv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Wolbachia_endosymbiont_of_Culex_pipiens Wolbachia endosymbiont of Culex pipiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FIV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FIV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.59&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fiv OCA], [https://pdbe.org/7fiv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fiv RCSB], [https://www.ebi.ac.uk/pdbsum/7fiv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fiv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2K9VS01_9RICK A0A2K9VS01_9RICK] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cytoplasmic incompatibility (CI) results when Wolbachia bacteria-infected male insects mate with uninfected females, leading to embryonic lethality. "Rescue" of viability occurs if the female harbors the same Wolbachia strain. CI is caused by linked pairs of Wolbachia genes called CI factors (CifA and CifB). The co-evolution of CifA-CifB pairs may account in part for the incompatibility patterns documented in insects infected with different Wolbachia strains, but the molecular mechanisms remain elusive. Here, we use X-ray crystallography and AlphaFold to analyze the CI factors from Wolbachia strain wMel called CidA(wMel) and CidB(wMel). Substituting CidA(wMel) interface residues with those from CidA(wPip) (from strain wPip) enables the mutant protein to bind CidB(wPip) and rescue CidB(wPip)-induced yeast growth defects, supporting the importance of CifA-CifB interaction in CI rescue. Sequence divergence in CidA(wPip) and CidB(wPip) proteins affects their pairwise interactions, which may help explain the complex incompatibility patterns of mosquitoes infected with different wPip strains.

Crystal Structures of Wolbachia CidA and CidB Reveal Determinants of Bacteria-induced Cytoplasmic Incompatibility and Rescue.,Wang H, Xiao Y, Chen X, Zhang M, Sun G, Wang F, Wang L, Zhang H, Zhang X, Yang X, Li W, Wei Y, Yao D, Zhang B, Li J, Cui W, Wang F, Chen C, Shen W, Su D, Bai F, Huang J, Ye S, Zhang L, Ji X, Wang W, Wang Z, Hochstrasser M, Yang H Nat Commun. 2022 Mar 25;13(1):1608. doi: 10.1038/s41467-022-29273-w. PMID:35338130<ref>PMID:35338130</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7fiv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Wolbachia endosymbiont of Culex pipiens]]
[[Category: Chen X]]
[[Category: Ji XY]]
[[Category: Wang W]]
[[Category: Xiao YJ]]
[[Category: Yang HT]]