View source for 7h57


==Crystal structure of endothiapepsin PF_cryo1 in complex with AC39729 at 100 K==
<StructureSection load='7h57' size='340' side='right'caption='[[7h57]], [[Resolution|resolution]] 1.99&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7h57]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cryphonectria_parasitica Cryphonectria parasitica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7H57 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7H57 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.988&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=U1Q:5-fluoranylpyridin-2-amine'>U1Q</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7h57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7h57 OCA], [https://pdbe.org/7h57 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7h57 RCSB], [https://www.ebi.ac.uk/pdbsum/7h57 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7h57 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CARP_CRYPA CARP_CRYPA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Advances in structural biology have relied heavily on synchrotron cryo-crystallography and cryogenic electron microscopy to elucidate biological processes and for drug discovery. However, disparities between cryogenic and room-temperature (RT) crystal structures pose challenges. Here, Cryo2RT, a high-throughput RT data-collection method from cryo-cooled crystals that leverages the cryo-crystallography workflow, is introduced. Tested on endothiapepsin crystals with four soaked fragments, thaumatin and SARS-CoV-2 3CL(pro), Cryo2RT reveals unique ligand-binding poses, offers a comparable throughput to cryo-crystallography and eases the exploration of structural dynamics at various temperatures.

Cryo2RT: a high-throughput method for room-temperature macromolecular crystallography from cryo-cooled crystals.,Huang CY, Aumonier S, Olieric V, Wang M Acta Crystallogr D Struct Biol. 2024 Aug 1;80(Pt 8):620-628. doi: , 10.1107/S2059798324006697. Epub 2024 Jul 25. PMID:39052318<ref>PMID:39052318</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7h57" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Cryphonectria parasitica]]
[[Category: Large Structures]]
[[Category: Aumonier S]]
[[Category: Huang C-Y]]
[[Category: Olieric V]]
[[Category: Wang M]]