View source for 7jgj


==IgA1 Protease in complex with neutralizing mAb==
<StructureSection load='7jgj' size='340' side='right'caption='[[7jgj]], [[Resolution|resolution]] 4.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7jgj]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JGJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JGJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jgj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jgj OCA], [https://pdbe.org/7jgj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jgj RCSB], [https://www.ebi.ac.uk/pdbsum/7jgj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jgj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IGA1_STRR6 IGA1_STRR6] Zinc metalloproteinase which cleaves human immunoglobulin A1 (IgA1) in the hinge region.<ref>PMID:8926056</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Opportunistic pathogens such as Streptococcus pneumoniae secrete a giant metalloprotease virulence factor responsible for cleaving host IgA1, yet the molecular mechanism has remained unknown since their discovery nearly 30 years ago despite the potential for developing vaccines that target these enzymes to block infection. Here we show through a series of cryo-electron microscopy single particle reconstructions how the Streptococcus pneumoniae IgA1 protease facilitates IgA1 substrate recognition and how this can be inhibited. Specifically, the Streptococcus pneumoniae IgA1 protease subscribes to an active-site-gated mechanism where a domain undergoes a 10.0 A movement to facilitate cleavage. Monoclonal antibody binding inhibits this conformational change, providing a direct means to block infection at the host interface. These structural studies explain decades of biological and biochemical studies and provides a general strategy to block Streptococcus pneumoniae IgA1 protease activity to potentially prevent infection.

Mechanism and inhibition of Streptococcus pneumoniae IgA1 protease.,Wang Z, Rahkola J, Redzic JS, Chi YC, Tran N, Holyoak T, Zheng H, Janoff E, Eisenmesser E Nat Commun. 2020 Nov 27;11(1):6063. doi: 10.1038/s41467-020-19887-3. PMID:33247098<ref>PMID:33247098</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7jgj" style="background-color:#fffaf0;"></div>

==See Also==
*[[Antibody 3D structures|Antibody 3D structures]]
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Streptococcus pneumoniae]]
[[Category: Eisenmesser EZ]]
[[Category: Zheng H]]