View source for 7jhy


==Type IV-B CRISPR Complex==
<StructureSection load='7jhy' size='340' side='right'caption='[[7jhy]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7jhy]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_sp._JS623 Mycobacterium sp. JS623]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JHY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JHY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jhy OCA], [https://pdbe.org/7jhy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jhy RCSB], [https://www.ebi.ac.uk/pdbsum/7jhy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jhy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/L0JA79_9MYCO L0JA79_9MYCO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We reveal the cryo-electron microscopy structure of a type IV-B CRISPR ribonucleoprotein (RNP) complex (Csf) at 3.9-A resolution. The complex best resembles the type III-A CRISPR Csm effector complex, consisting of a Cas7-like (Csf2) filament intertwined with a small subunit (Cas11) filament, but the complex lacks subunits for RNA processing and target DNA cleavage. Surprisingly, instead of assembling around a CRISPR-derived RNA (crRNA), the complex assembles upon heterogeneous RNA of a regular length arranged in a pseudo-A-form configuration. These findings provide a high-resolution glimpse into the assembly and function of enigmatic type IV CRISPR systems, expanding our understanding of class I CRISPR-Cas system architecture, and suggesting a function for type IV-B RNPs that may be distinct from other class 1 CRISPR-associated systems.

Structure of a type IV CRISPR-Cas ribonucleoprotein complex.,Zhou Y, Bravo JPK, Taylor HN, Steens JA, Jackson RN, Staals RHJ, Taylor DW iScience. 2021 Feb 17;24(3):102201. doi: 10.1016/j.isci.2021.102201. eCollection , 2021 Mar 19. PMID:33733066<ref>PMID:33733066</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7jhy" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycobacterium sp. JS623]]
[[Category: Bravo JPK]]
[[Category: Taylor DW]]