View source for 7jj8


==Crystal structure of the Zn(II)-bound ZnuA-like domain of Streptococcus pneumoniae AdcA==
<StructureSection load='7jj8' size='340' side='right'caption='[[7jj8]], [[Resolution|resolution]] 2.03&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7jj8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_R6 Streptococcus pneumoniae R6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JJ8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jj8 OCA], [https://pdbe.org/7jj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jj8 RCSB], [https://www.ebi.ac.uk/pdbsum/7jj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jj8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ADCA_STRR6 ADCA_STRR6] Part of the ATP-driven transport system AdcABC for zinc. Required for transformability.<ref>PMID:9379902</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Zinc is an essential element in all domains of life. Nonetheless, how prokaryotes achieve selective acquisition of zinc from the extracellular environment remains poorly understood. Here, we elucidate a novel mechanism for zinc-binding in AdcA, a solute-binding protein of Streptococcus pneumoniae Crystal structure analyses reveal the two-domain organization of the protein and show that only the N-terminal domain (AdcAN) is necessary for zinc import. Zinc binding induces only minor changes in the global protein conformation of AdcA and stabilizes a highly mobile loop within the AdcAN domain. This loop region, which is conserved in zinc-specific solute-binding proteins, facilitates closure of the AdcAN binding site and is crucial for zinc acquisition. Collectively, these findings elucidate the structural and functional basis of selective zinc uptake in prokaryotes.IMPORTANCE Zinc is an essential nutrient for the virulence of bacterial pathogens such as Streptococcus pneumoniae Many Gram-positive bacteria use a two-domain lipoprotein for zinc acquisition, but how this class of metal-recruiting proteins acquire zinc and interact with the uptake machinery has remained poorly defined. We report the first structure of a two-domain lipoprotein, AdcA from S. pneumoniae, and use computational, spectroscopic, and microbiological approaches to provide new insights into the functional basis of zinc recruitment. Our findings reveal that AdcA employs a novel mechanism for zinc binding that we have termed the "trap-door" mechanism, and we show how the static metal-binding site of the protein, which confers its selectivity for zinc ions, is combined with a dynamic surface element to facilitate zinc recruitment and import into the bacterium. Together, these findings expand our understanding of how bacteria acquire zinc from the environment and provide a foundation for inhibiting this process, through antimicrobial targeting of the dynamic structural elements to block bacterial zinc scavenging.

A Trap-Door Mechanism for Zinc Acquisition by Streptococcus pneumoniae AdcA.,Luo Z, Morey JR, Deplazes E, Motygullina A, Tan A, Ganio K, Neville SL, Eleftheriadis N, Isselstein M, Pederick VG, Paton JC, Cordes T, Harmer JR, Kobe B, McDevitt CA mBio. 2021 Feb 2;12(1). pii: mBio.01958-20. doi: 10.1128/mBio.01958-20. PMID:33531394<ref>PMID:33531394</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7jj8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptococcus pneumoniae R6]]
[[Category: Kobe B]]
[[Category: Luo Z]]
[[Category: McDevitt CA]]
[[Category: More JR]]