View source for 7jmc


==Sheep Connexin-50 at 2.5 angstroms resolution, Lipid Class 3==
<StructureSection load='7jmc' size='340' side='right'caption='[[7jmc]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JMC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JMC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MC3:1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE'>MC3</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jmc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jmc OCA], [https://pdbe.org/7jmc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jmc RCSB], [https://www.ebi.ac.uk/pdbsum/7jmc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jmc ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Gap junctions establish direct pathways for cells to transfer metabolic and electrical messages. The local lipid environment is known to affect the structure, stability and intercellular channel activity of gap junctions; however, the molecular basis for these effects remains unknown. Here, we incorporate native connexin-46/50 (Cx46/50) intercellular channels into a dual lipid nanodisc system, mimicking a native cell-to-cell junction. Structural characterization by CryoEM reveals a lipid-induced stabilization to the channel, resulting in a 3D reconstruction at 1.9 A resolution. Together with all-atom molecular dynamics simulations, it is shown that Cx46/50 in turn imparts long-range stabilization to the dynamic local lipid environment that is specific to the extracellular lipid leaflet. In addition, ~400 water molecules are resolved in the CryoEM map, localized throughout the intercellular permeation pathway and contributing to the channel architecture. These results illustrate how the aqueous-lipid environment is integrated with the architectural stability, structure and function of gap junction communication channels.

Connexin-46/50 in a dynamic lipid environment resolved by CryoEM at 1.9 A.,Flores JA, Haddad BG, Dolan KA, Myers JB, Yoshioka CC, Copperman J, Zuckerman DM, Reichow SL Nat Commun. 2020 Aug 28;11(1):4331. doi: 10.1038/s41467-020-18120-5. PMID:32859914<ref>PMID:32859914</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 7jmc" style="background-color:#fffaf0;"></div>

==See Also==
*[[Connexin 3D structure|Connexin 3D structure]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Copperman J]]
[[Category: Dolan KD]]
[[Category: Flores JA]]
[[Category: Haddad BG]]
[[Category: Myers JB]]
[[Category: Reichow SL]]
[[Category: Yoshioka CC]]
[[Category: Zuckerman DM]]