View source for 7joq


==Structure of NV1 small terminase==
<StructureSection load='7joq' size='340' side='right'caption='[[7joq]], [[Resolution|resolution]] 3.95&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7joq]] is a 81 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_phage_NV1 Pseudomonas phage NV1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JOQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JOQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.95&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7joq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7joq OCA], [https://pdbe.org/7joq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7joq RCSB], [https://www.ebi.ac.uk/pdbsum/7joq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7joq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2L0HPR5_9CAUD A0A2L0HPR5_9CAUD] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The genome packaging motor of tailed bacteriophages and herpesviruses is a powerful nanomachine built by several copies of a large (TerL) and a small (TerS) terminase subunit. The motor assembles transiently at the portal vertex of an empty precursor capsid (or procapsid) to power genome encapsidation. Terminase subunits have been studied in-depth, especially in classical bacteriophages that infect Escherichia coli or Salmonella, yet, less is known about the packaging motor of Pseudomonas-phages that have increasing biomedical relevance. Here, we investigated the small terminase subunit from three Podoviridae phages that infect Pseudomonas aeruginosa. We found TerS is polymorphic in solution but assembles into a nonamer in its high-affinity heparin-binding conformation. The atomic structure of Pseudomonas phage PaP3 TerS, the first complete structure for a TerS from a cos phage, reveals nine helix-turn-helix (HTH) motifs asymmetrically arranged around a beta-stranded channel, too narrow to accommodate DNA. PaP3 TerS binds DNA in a sequence-specific manner in vitro. X-ray scattering and molecular modeling suggest TerS adopts an open conformation in solution, characterized by dynamic HTHs that move around an oligomerization core, generating discrete binding crevices for DNA. We propose a model for sequence-specific recognition of packaging initiation sites by lateral interdigitation of DNA.

Biophysical analysis of Pseudomonas-phage PaP3 small terminase suggests a mechanism for sequence-specific DNA-binding by lateral interdigitation.,Niazi M, Florio TJ, Yang R, Lokareddy RK, Swanson NA, Gillilan RE, Cingolani G Nucleic Acids Res. 2020 Oct 30. pii: 5943822. doi: 10.1093/nar/gkaa866. PMID:33125059<ref>PMID:33125059</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7joq" style="background-color:#fffaf0;"></div>

==See Also==
*[[Terminase 3D Structures|Terminase 3D Structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas phage NV1]]
[[Category: Cingolani G]]
[[Category: Lokareddy R]]