View source for 7kbf


==H1.8 bound nucleosome isolated from metaphase chromosome in Xenopus egg extract (oligo fraction)==
<StructureSection load='7kbf' size='340' side='right'caption='[[7kbf]], [[Resolution|resolution]] 4.42&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7kbf]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KBF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.42&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kbf OCA], [https://pdbe.org/7kbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kbf RCSB], [https://www.ebi.ac.uk/pdbsum/7kbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kbf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B4_XENLA B4_XENLA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Structural heterogeneity of nucleosomes in functional chromosomes is unknown. Here, we devise the template-, reference- and selection-free (TRSF) cryo-EM pipeline to simultaneously reconstruct cryo-EM structures of protein complexes from interphase or metaphase chromosomes. The reconstructed interphase and metaphase nucleosome structures are on average indistinguishable from canonical nucleosome structures, despite DNA sequence heterogeneity, cell-cycle-specific posttranslational modifications, and interacting proteins. Nucleosome structures determined by a decoy-classifying method and structure variability analyses reveal the nucleosome structural variations in linker DNA, histone tails, and nucleosome core particle configurations, suggesting that the opening of linker DNA, which is correlated with H2A C-terminal tail positioning, is suppressed in chromosomes. High-resolution (3.4-3.5 A) nucleosome structures indicate DNA-sequence-independent stabilization of superhelical locations +/-0-1 and +/-3.5-4.5. The linker histone H1.8 preferentially binds to metaphase chromatin, from which chromatosome cryo-EM structures with H1.8 at the on-dyad position are reconstituted. This study presents the structural characteristics of nucleosomes in chromosomes.

Structural features of nucleosomes in interphase and metaphase chromosomes.,Arimura Y, Shih RM, Froom R, Funabiki H Mol Cell. 2021 Nov 4;81(21):4377-4397.e12. doi: 10.1016/j.molcel.2021.08.010. , Epub 2021 Sep 2. PMID:34478647<ref>PMID:34478647</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7kbf" style="background-color:#fffaf0;"></div>

==See Also==
*[[Histone 3D structures|Histone 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Xenopus laevis]]
[[Category: Arimura Y]]
[[Category: Funabiki H]]