View source for 7kiy


==Plasmodium falciparum RhopH complex in soluble form==
<StructureSection load='7kiy' size='340' side='right'caption='[[7kiy]], [[Resolution|resolution]] 2.92&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7kiy]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KIY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KIY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.92&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kiy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kiy OCA], [https://pdbe.org/7kiy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kiy RCSB], [https://www.ebi.ac.uk/pdbsum/7kiy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kiy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RCH1B_PLAFA RCH1B_PLAFA] Participates in the formation of new permeability pathways in Plasmodium-infected erythrocytes enabling the uptake of nutrients from the blood plasma.[UniProtKB:A0A1W6IZJ5]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Malaria parasites use the RhopH complex for erythrocyte invasion and channel-mediated nutrient uptake. As the member proteins are unique to Plasmodium spp., how they interact and traffic through subcellular sites to serve these essential functions is unknown. We show that RhopH is synthesized as a soluble complex of CLAG3, RhopH2, and RhopH3 with 1:1:1 stoichiometry. After transfer to a new host cell, the complex crosses a vacuolar membrane surrounding the intracellular parasite and becomes integral to the erythrocyte membrane through a PTEX translocon-dependent process. We present a 2.9 A single-particle cryo-electron microscopy structure of the trafficking complex, revealing that CLAG3 interacts with the other subunits over large surface areas. This soluble complex is tightly assembled with extensive disulfide bonding and predicted transmembrane helices shielded. We propose a large protein complex stabilized for trafficking but poised for host membrane insertion through large-scale rearrangements, paralleling smaller two-state pore-forming proteins in other organisms.

Malaria parasites use a soluble RhopH complex for erythrocyte invasion and an integral form for nutrient uptake.,Schureck MA, Darling JE, Merk A, Shao J, Daggupati G, Srinivasan P, Olinares PDB, Rout MP, Chait BT, Wollenberg K, Subramaniam S, Desai SA Elife. 2021 Jan 4;10:e65282. doi: 10.7554/eLife.65282. PMID:33393463<ref>PMID:33393463</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7kiy" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Plasmodium falciparum]]
[[Category: Darling JE]]
[[Category: Desai SA]]
[[Category: Merk A]]
[[Category: Schureck MA]]
[[Category: Subramaniam S]]