View source for 7ksf


==Crystal structure of Prototype Foamy Virus Protease-Reverse Transcriptase (native)==
<StructureSection load='7ksf' size='340' side='right'caption='[[7ksf]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ksf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Eastern_chimpanzee_simian_foamy_virus Eastern chimpanzee simian foamy virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KSF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KSF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ksf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ksf OCA], [https://pdbe.org/7ksf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ksf RCSB], [https://www.ebi.ac.uk/pdbsum/7ksf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ksf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A1Q1N9V8_9RETR A0A1Q1N9V8_9RETR] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In most cases, proteolytic processing of the retroviral Pol portion of the Gag-Pol polyprotein precursor produces protease (PR), reverse transcriptase (RT), and integrase (IN). However, foamy viruses (FVs) express Pol separately from Gag and, when Pol is processed, only the IN domain is released. Here, we report a 2.9 A resolution crystal structure of the mature PR-RT from prototype FV (PFV) that can carry out both proteolytic processing and reverse transcription but is in a configuration not competent for proteolytic or polymerase activity. PFV PR-RT is monomeric and the architecture of PFV PR is similar to one of the subunits of HIV-1 PR, which is a dimer. There is a C-terminal extension of PFV PR (101-145) that consists of two helices which are adjacent to the base of the RT palm subdomain, and anchors PR to RT. The polymerase domain of PFV RT consists of fingers, palm, thumb, and connection subdomains whose spatial arrangements are similar to the p51 subunit of HIV-1 RT. The RNase H and polymerase domains of PFV RT are connected by flexible linkers. Significant spatial and conformational (sub)domain rearrangements are therefore required for nucleic acid binding. The structure of PFV PR-RT provides insights into the conformational maturation of retroviral Pol polyproteins.

Crystal Structure of a Retroviral Polyprotein: Prototype Foamy Virus Protease-Reverse Transcriptase (PR-RT).,Harrison JJEK, Tuske S, Das K, Ruiz FX, Bauman JD, Boyer PL, DeStefano JJ, Hughes SH, Arnold E Viruses. 2021 Jul 29;13(8). pii: v13081495. doi: 10.3390/v13081495. PMID:34452360<ref>PMID:34452360</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ksf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Eastern chimpanzee simian foamy virus]]
[[Category: Large Structures]]
[[Category: Arnold E]]
[[Category: Das K]]
[[Category: Harrison JJEK]]
[[Category: Ruiz FX]]