View source for 7lqh


==Cryo-EM of KFE8 thinner nanotube (class 2, 2-sub-1)==
<StructureSection load='7lqh' size='340' side='right'caption='[[7lqh]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7lqh]] is a 144 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LQH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LQH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5CR:N-ACETYL-L-PHENYLALANINE'>5CR</scene>, <scene name='pdbligand=GMA:4-AMIDO-4-CARBAMOYL-BUTYRIC+ACID'>GMA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lqh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lqh OCA], [https://pdbe.org/7lqh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lqh RCSB], [https://www.ebi.ac.uk/pdbsum/7lqh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lqh ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The self-assembly of designed peptides into filaments and other higher-order structures has been the focus of intense interest because of the potential for creating new biomaterials and biomedical devices. These peptide assemblies have also been used as models for understanding biological processes, such as the pathological formation of amyloid. We investigate the assembly of an octapeptide sequence, Ac-FKFEFKFE-NH2, motivated by prior studies that demonstrated that this amphipathic beta strand peptide self-assembled into fibrils and biocompatible hydrogels. Using high-resolution cryoelectron microscopy (cryo-EM), we are able to determine the atomic structure for two different coexisting forms of the fibrils, containing four and five beta sandwich protofilaments, respectively. Surprisingly, the inner walls in both forms are parallel beta sheets, while the outer walls are antiparallel beta sheets. Our results demonstrate the chaotic nature of peptide self-assembly and illustrate the importance of cryo-EM structural analysis to understand the complex phase behavior of these materials at near-atomic resolution.

Deterministic chaos in the self-assembly of beta sheet nanotubes from an amphipathic oligopeptide.,Wang F, Gnewou O, Wang S, Osinski T, Zuo X, Egelman EH, Conticello VP Matter. 2021 Oct 6;4(10):3217-3231. doi: 10.1016/j.matt.2021.06.037. Epub 2021, Jul 27. PMID:34632372<ref>PMID:34632372</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7lqh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Conticello VP]]
[[Category: Egelman EH]]
[[Category: Gnewou OM]]
[[Category: Wang F]]