View source for 7m58


==Crystal structure of N1, a member of cis-3-chloroacrylic acid dehalogenase (cis-CaaD) family==
<StructureSection load='7m58' size='340' side='right'caption='[[7m58]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7m58]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_halotolerans_YIM_70093_=_DSM_44683 Corynebacterium halotolerans YIM 70093 = DSM 44683]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7M58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7M58 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m58 OCA], [https://pdbe.org/7m58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m58 RCSB], [https://www.ebi.ac.uk/pdbsum/7m58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m58 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/M1NLA4_9CORY M1NLA4_9CORY] 
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== Publication Abstract from PubMed ==
The tautomerase superfamily (TSF) is a collection of enzymes and proteins that share a simple beta-alpha-beta structural scaffold. Most members are constructed from a single-core beta-alpha-beta motif or two consecutively fused beta-alpha-beta motifs in which the N-terminal proline (Pro-1) plays a key and unusual role as a catalytic residue. The cumulative evidence suggests that a gene fusion event took place in the evolution of the TSF followed by duplication (of the newly fused gene) to result in the diversification of activity that is seen today. Analysis of the sequence similarity network (SSN) for the TSF identified several linking proteins ("linkers") whose similarity links subgroups of these contemporary proteins that might hold clues about structure-function relationship changes accompanying the emergence of new activities. A previously uncharacterized pair of linkers (designated N1 and N2) was identified in the SSN that connected the 4-oxalocrotonate tautomerase (4-OT) and cis-3-chloroacrylic acid dehalogenase (cis-CaaD) subgroups. N1, in the cis-CaaD subgroup, has the full complement of active site residues for cis-CaaD activity, whereas N2, in the 4-OT subgroup, lacks a key arginine (Arg-39) for canonical 4-OT activity. Kinetic characterization and nuclear magnetic resonance analysis show that N1 has activities observed for other characterized members of the cis-CaaD subgroup with varying degrees of efficiencies. N2 is a modest 4-OT but shows enhanced hydratase activity using allene and acetylene compounds, which might be due to the presence of Arg-8 along with Arg-11. Crystallographic analysis provides a structural context for these observations.

Kinetic and Structural Analysis of Two Linkers in the Tautomerase Superfamily: Analysis and Implications.,Baas BJ, Medellin BP, LeVieux JA, Erwin K, Lancaster EB, Johnson WH Jr, Kaoud TS, Moreno RY, de Ruijter M, Babbitt PC, Zhang YJ, Whitman CP Biochemistry. 2021 May 21. doi: 10.1021/acs.biochem.1c00220. PMID:34019384<ref>PMID:34019384</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 7m58" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Corynebacterium halotolerans YIM 70093 = DSM 44683]]
[[Category: Large Structures]]
[[Category: Medellin BP]]
[[Category: Moreno RY]]
[[Category: Zhang YJ]]