View source for 7m8q


==Complex structure of Methane monooxygenase hydroxylase and regulatory subunit with fluorosubstituted tryptophans==
<StructureSection load='7m8q' size='340' side='right'caption='[[7m8q]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7m8q]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylosinus_trichosporium_OB3b Methylosinus trichosporium OB3b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7M8Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7M8Q FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.08&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FTR:FLUOROTRYPTOPHANE'>FTR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m8q OCA], [https://pdbe.org/7m8q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m8q RCSB], [https://www.ebi.ac.uk/pdbsum/7m8q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m8q ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MEMA_METTR MEMA_METTR] Responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyzes the monohydroxylation of a variety of unactivated alkenes, alicyclic, aromatic and heterocyclic compounds.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Soluble methane monooxygenase (sMMO) is a multicomponent metalloenzyme capable of catalyzing the fissure of the C-H bond of methane and the insertion of one atom of oxygen from O(2) to yield methanol. Efficient multiple-turnover catalysis occurs only in the presence of all three sMMO protein components: hydroxylase (MMOH), reductase (MMOR), and regulatory protein (MMOB). The complex series of sMMO protein component interactions that regulate the formation and decay of sMMO reaction cycle intermediates is not fully understood. Here, the two tryptophan residues in MMOB and the single tryptophan residue in MMOR are converted to 5-fluorotryptophan (5FW) by expression in defined media containing 5-fluoroindole. In addition, the mechanistically significant N-terminal region of MMOB is (19)F-labeled by reaction of the K15C variant with 3-bromo-1,1,1-trifluoroacetone (BTFA). The 5FW and BTFA modifications cause minimal structural perturbation, allowing detailed studies of the interactions with sMMOH using (19)F NMR. Resonances from the 275 kDa complexes of sMMOH with 5FW-MMOB and BTFA-K15C-5FW-MMOB are readily detected at 5 muM labeled protein concentration. This approach shows directly that MMOR and MMOB competitively bind to sMMOH with similar K(D) values, independent of the oxidation state of the sMMOH diiron cluster. These findings suggest a new model for regulation in which the dynamic equilibration of MMOR and MMOB with sMMOH allows a transient formation of key reactive complexes that irreversibly pull the reaction cycle forward. The slow kinetics of exchange of the sMMOH:MMOB complex is proposed to prevent MMOR-mediated reductive quenching of the high-valent reaction cycle intermediate Q before it can react with methane.

Soluble Methane Monooxygenase Component Interactions Monitored by (19)F NMR.,Jones JC, Banerjee R, Shi K, Semonis MM, Aihara H, Pomerantz WCK, Lipscomb JD Biochemistry. 2021 Jun 29;60(25):1995-2010. doi: 10.1021/acs.biochem.1c00293. , Epub 2021 Jun 8. PMID:34100595<ref>PMID:34100595</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 7m8q" style="background-color:#fffaf0;"></div>

==See Also==
*[[Methane monooxygenase 3D structures|Methane monooxygenase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methylosinus trichosporium OB3b]]
[[Category: Aihara H]]
[[Category: Banerjee R]]
[[Category: Johns JC]]
[[Category: Lipscomb JD]]
[[Category: Pomerantz WCK]]
[[Category: Semonis MM]]
[[Category: Shi K]]