View source for 7mf3


==Structure of the autoinhibited state of smooth muscle myosin-2==
<StructureSection load='7mf3' size='340' side='right'caption='[[7mf3]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7mf3]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MF3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mf3 OCA], [https://pdbe.org/7mf3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mf3 RCSB], [https://www.ebi.ac.uk/pdbsum/7mf3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mf3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MYH11_CHICK MYH11_CHICK] Muscle contraction.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We solved the near-atomic resolution structure of smooth muscle myosin-2 in the autoinhibited state (10S) using single-particle cryo-electron microscopy. The 3.4-A structure reveals the precise molecular architecture of 10S and the structural basis for myosin-2 regulation. We reveal the position of the phosphorylation sites that control myosin autoinhibition and activation by phosphorylation of the regulatory light chain. Further, we present a previously unidentified conformational state in myosin-2 that traps ADP and P(i) produced by the hydrolysis of ATP in the active site. This noncanonical state represents a branch of the myosin enzyme cycle and explains the autoinhibition of the enzyme function of 10S along with its reduced affinity for actin. Together, our structure defines the molecular mechanisms that drive 10S formation, stabilization, and relief by phosphorylation of the regulatory light chain.

Cryo-EM structure of the autoinhibited state of myosin-2.,Heissler SM, Arora AS, Billington N, Sellers JR, Chinthalapudi K Sci Adv. 2021 Dec 24;7(52):eabk3273. doi: 10.1126/sciadv.abk3273. Epub 2021 Dec , 22. PMID:34936462<ref>PMID:34936462</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7mf3" style="background-color:#fffaf0;"></div>

==See Also==
*[[Myosin 3D Structures|Myosin 3D Structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Gallus gallus]]
[[Category: Large Structures]]
[[Category: Arora AS]]
[[Category: Billington N]]
[[Category: Chinthalapudi K]]
[[Category: Heissler SM]]
[[Category: Sellers JR]]