View source for 7mh2


==T4GALA Engineered Protein Nanocage==
<StructureSection load='7mh2' size='340' side='right'caption='[[7mh2]], [[Resolution|resolution]] 3.57&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MH2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MH2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.57&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mh2 OCA], [https://pdbe.org/7mh2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mh2 RCSB], [https://www.ebi.ac.uk/pdbsum/7mh2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mh2 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Protein nanocages play crucial roles in sub-cellular compartmentalization and spatial control in all domains of life and have been used as biomolecular tools for applications in biocatalysis, drug delivery, and bionanotechnology. The ability to control their assembly state under physiological conditions would further expand their practical utility. To gain such control, we introduced a peptide capable of triggering conformational change at a key structural position in the largest known encapsulin nanocompartment. We report the structure of the resulting engineered nanocage and demonstrate its ability to disassemble and reassemble on demand under physiological conditions. We demonstrate its capacity for in vivo encapsulation of proteins of choice while also demonstrating in vitro cargo loading capabilities. Our results represent a functionally robust addition to the nanocage toolbox and a novel approach for controlling protein nanocage disassembly and reassembly under mild conditions.

Triggered reversible disassembly of an engineered protein nanocage.,Giessen TW, Jones JA, Cristie-David AS, Andreas MP Angew Chem Int Ed Engl. 2021 Sep 16. doi: 10.1002/anie.202110318. PMID:34532937<ref>PMID:34532937</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7mh2" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Andreas MP]]
[[Category: Cristie-David AS]]
[[Category: Giessen TW]]
[[Category: Jones JA]]