View source for 7msl


==Structure of anti-CRISPR AcrIIC4 from Haemophilus parainfluenzae==
<StructureSection load='7msl' size='340' side='right'caption='[[7msl]], [[Resolution|resolution]] 1.73&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7msl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_parainfluenzae Haemophilus parainfluenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MSL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.73&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7msl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7msl OCA], [https://pdbe.org/7msl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7msl RCSB], [https://www.ebi.ac.uk/pdbsum/7msl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7msl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A377JKY9_HAEPA A0A377JKY9_HAEPA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Phages, plasmids, and other mobile genetic elements express inhibitors of CRISPR-Cas immune systems, known as anti-CRISPR proteins, to protect themselves from targeted destruction. These anti-CRISPR proteins have been shown to function through very diverse mechanisms. In this work we investigate the activity of an anti-CRISPR isolated from a prophage in Haemophilus parainfluenzae that blocks CRISPR-Cas9 DNA cleavage activity. We determine the three-dimensional crystal structure of AcrIIC4Hpa and show that it binds to the Cas9 Recognition Domain. This binding does not prevent the Cas9-anti-CRISPR complex from interacting with target DNA but does inhibit DNA cleavage. AcrIIC4Hpa likely acts by blocking the conformational changes that allow the HNH and RuvC endonuclease domains to contact the DNA sites to be nicked.

Structural and Mechanistic Insight into CRISPR-Cas9 Inhibition by Anti-CRISPR Protein AcrIIC4Hpa.,Hwang S, Pan C, Garcia B, Davidson AR, Moraes TF, Maxwell KL J Mol Biol. 2022 Mar 15;434(5):167420. doi: 10.1016/j.jmb.2021.167420. Epub 2021 , Dec 23. PMID:34954237<ref>PMID:34954237</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7msl" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Haemophilus parainfluenzae]]
[[Category: Large Structures]]
[[Category: Maxwell KL]]
[[Category: Moraes TF]]
[[Category: Pan C]]