View source for 7mue


==Legionella pneumophila Dot/Icm T4SS PR==
<StructureSection load='7mue' size='340' side='right'caption='[[7mue]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7mue]] is a 72 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MUE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MUE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mue OCA], [https://pdbe.org/7mue PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mue RCSB], [https://www.ebi.ac.uk/pdbsum/7mue PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mue ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O54615_LEGPN O54615_LEGPN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Legionella pneumophila is an opportunistic pathogen that causes the potentially fatal pneumonia known as Legionnaires' disease. The pathology associated with infection depends on bacterial delivery of effector proteins into the host via the membrane spanning Dot/Icm type IV secretion system (T4SS). We have determined sub-3.0 A resolution maps of the Dot/Icm T4SS core complex by single particle cryo-EM. The high-resolution structural analysis has allowed us to identify proteins encoded outside the Dot/Icm genetic locus that contribute to the core T4SS structure. We can also now define two distinct areas of symmetry mismatch, one that connects the C18 periplasmic ring (PR) and the C13 outer membrane cap (OMC) and one that connects the C13 OMC with a 16-fold symmetric dome. Unexpectedly, the connection between the PR and OMC is DotH, with five copies sandwiched between the OMC and PR to accommodate the symmetry mismatch. Finally, we observe multiple conformations in the reconstructions that indicate flexibility within the structure.

Cryo-EM reveals new species-specific proteins and symmetry elements in the Legionella pneumophila Dot/Icm T4SS.,Sheedlo MJ, Durie CL, Chung JM, Chang L, Roberts J, Swanson M, Lacy DB, Ohi MD Elife. 2021 Sep 14;10:e70427. doi: 10.7554/eLife.70427. PMID:34519271<ref>PMID:34519271</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7mue" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Legionella pneumophila]]
[[Category: Durie CL]]
[[Category: Lacy DB]]
[[Category: Ohi MD]]
[[Category: Sheedlo MJ]]
[[Category: Swanson M]]