View source for 7n85


==Inner ring spoke from the isolated yeast NPC==
<SX load='7n85' size='340' side='right' viewer='molstar' caption='[[7n85]], [[Resolution|resolution]] 7.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7n85]] is a 28 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N85 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N85 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n85 OCA], [https://pdbe.org/7n85 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n85 RCSB], [https://www.ebi.ac.uk/pdbsum/7n85 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n85 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NUP59_YEAST NUP59_YEAST] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). May have a mitosis control function (By similarity).<ref>PMID:10684247</ref> <ref>PMID:12604785</ref> <ref>PMID:9864357</ref> 
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== Publication Abstract from PubMed ==
Nuclear pore complexes (NPCs) mediate the nucleocytoplasmic transport of macromolecules. Here we provide a structure of the isolated yeast NPC in which the inner ring is resolved by cryo-EM at sub-nanometer resolution to show how flexible connectors tie together different structural and functional layers. These connectors may be targets for phosphorylation and regulated disassembly in cells with an open mitosis. Moreover, some nucleoporin pairs and transport factors have similar interaction motifs, which suggests an evolutionary and mechanistic link between assembly and transport. We provide evidence for three major NPC variants that may foreshadow functional specializations at the nuclear periphery. Cryo-electron tomography extended these studies, providing a model of the in situ NPC with a radially expanded inner ring. Our comprehensive model reveals features of the nuclear basket and central transporter, suggests a role for the lumenal Pom152 ring in restricting dilation, and highlights structural plasticity that may be required for transport.

Comprehensive structure and functional adaptations of the yeast nuclear pore complex.,Akey CW, Singh D, Ouch C, Echeverria I, Nudelman I, Varberg JM, Yu Z, Fang F, Shi Y, Wang J, Salzberg D, Song K, Xu C, Gumbart JC, Suslov S, Unruh J, Jaspersen SL, Chait BT, Sali A, Fernandez-Martinez J, Ludtke SJ, Villa E, Rout MP Cell. 2022 Jan 20;185(2):361-378.e25. doi: 10.1016/j.cell.2021.12.015. Epub 2022 , Jan 3. PMID:34982960<ref>PMID:34982960</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 7n85" style="background-color:#fffaf0;"></div>

==See Also==
*[[Nucleoporin 3D structures|Nucleoporin 3D structures]]
== References ==
<references/>
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</SX>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Akey CW]]
[[Category: Echevarria I]]
[[Category: Fernandez-Martinez J]]
[[Category: Nudelman I]]
[[Category: Ouch C]]
[[Category: Rout MP]]