View source for 7n99


==SDE2 SAP domain apo structure==
<StructureSection load='7n99' size='340' side='right'caption='[[7n99]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7n99]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N99 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n99 OCA], [https://pdbe.org/7n99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n99 RCSB], [https://www.ebi.ac.uk/pdbsum/7n99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n99 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SDE2_HUMAN SDE2_HUMAN] Involved in both DNA replication and cell cycle control (PubMed:27906959). Unprocessed SDE2 interacts with PCNA via its PIP-box. The interaction with PCNA prevents monoubiquitination of the latter thereby inhibiting translesion DNA synthesis. The binding of SDE2 to PCNA also leads to processing of SDE2 by an unidentified deubiquitinating enzyme, cleaving off the N-terminal ubiquitin-like domain. The resulting mature SDE2 is degraded by the DCX(DTL) complex in a cell cycle- and DNA damage dependent manner (PubMed:27906959). Binding of SDE2 to PCNA is necessary to counteract damage due to ultraviolet light induced replication stress. The complete degradation of SDE2 is necessary to allow S-phase progression (PubMed:27906959).<ref>PMID:27906959</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Elevated DNA replication stress causes instability of the DNA replication fork and increased DNA mutations, which underlies tumorigenesis. The DNA replication stress regulator silencing-defective 2 (SDE2) is known to bind to TIMELESS (TIM), a protein of the fork protection complex, and enhances its stability, thereby supporting replisome activity at DNA replication forks. However, the DNA-binding activity of SDE2 is not well defined. Here, we structurally and functionally characterize a new conserved DNA-binding motif related to the SAP (SAF-A/B, Acinus, PIAS) domain in human SDE2 and establish its preference for ssDNA. Our NMR solution structure of the SDE2(SAP) domain reveals a helix-extended loop-helix core with the helices aligned parallel to each other, consistent with known canonical SAP folds. Notably, we have shown that the DNA interaction of this SAP domain extends beyond the core SAP domain and is augmented by two lysine residues in the C-terminal tail, which is uniquely positioned adjacent to the SAP motif and conserved in the pre-mRNA splicing factor SF3A3. Furthermore, we found that mutation in the SAP domain and extended C terminus not only disrupts ssDNA binding but also impairs TIM localization at replication forks, thus inhibiting efficient fork progression. Taken together, our results establish SDE2(SAP) as an essential element for SDE2 to exert its role in preserving replication fork integrity via fork protection complex regulation and highlight the structural diversity of the DNA-protein interactions achieved by a specialized DNA-binding motif.

Extended DNA-binding interfaces beyond the canonical SAP domain contribute to the function of replication stress regulator SDE2 at DNA replication forks.,Weinheimer AS, Paung Y, Rageul J, Khan A, Lo N, Ho B, Tong M, Alphonse S, Seeliger MA, Kim H J Biol Chem. 2022 Aug;298(8):102268. doi: 10.1016/j.jbc.2022.102268. Epub 2022 , Jul 16. PMID:35850305<ref>PMID:35850305</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7n99" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Alphonse S]]
[[Category: Ho B]]
[[Category: Khan A]]
[[Category: Kim H]]
[[Category: Paung Y]]
[[Category: Rageul J]]
[[Category: Seeliger MA]]
[[Category: Tong M]]
[[Category: Weinheimer AS]]