View source for 7nea


==Crystal structure of branched-chain amino acid aminotransferase from Thermobaculum terrenum (M3 mutant).==
<StructureSection load='7nea' size='340' side='right'caption='[[7nea]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7nea]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobaculum_terrenum_ATCC_BAA-798 Thermobaculum terrenum ATCC BAA-798]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NEA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NEA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nea OCA], [https://pdbe.org/7nea PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nea RCSB], [https://www.ebi.ac.uk/pdbsum/7nea PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nea ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D1CCW1_THET1 D1CCW1_THET1] Acts on leucine, isoleucine and valine.[RuleBase:RU364094]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Creating biocatalysts for (R)-selective amination effectively is highly desirable in organic synthesis. Despite noticeable progress in the engineering of (R)-amine activity in pyridoxal-5'-phosphate-dependent transaminases of fold type IV, the specialization of the activity is still an intuitive task, as there is poor understanding of sequence-structure-function relationships. In this study, we analyzed this relationship in transaminase from Thermobaculum terrenum, distinguished by expanded substrate specificity and activity in reactions with L-amino acids and (R)-(+)-1-phenylethylamine using alpha-ketoglutarate and pyruvate as amino acceptors. We performed site-directed mutagenesis to create a panel of the enzyme variants, which differ in the active site residues from the parent enzyme to a putative transaminase specific to (R)-primary amines. The variants were examined in the overall transamination reactions and half-reaction with (R)-(+)-1-phenylethylamine. A structural analysis of the most prominent variants revealed a spatial reorganization in the active sites, which caused changes in activity. Although the specialization to (R)-amine transaminase was not implemented, we succeeded in understanding the role of the particular active site residues in expanding substrate specificity of the enzyme. We showed that the specificity for (R)-(+)-1-phenylethylamine in transaminase from T. terrenum arises without sacrificing the specificity for L-amino acids and alpha-ketoglutarate and in consensus with it.

Probing the role of the residues in the active site of the transaminase from Thermobaculum terrenum.,Bezsudnova EY, Nikolaeva AY, Bakunova AK, Rakitina TV, Suplatov DA, Popov VO, Boyko KM PLoS One. 2021 Jul 29;16(7):e0255098. doi: 10.1371/journal.pone.0255098., eCollection 2021. PMID:34324538<ref>PMID:34324538</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7nea" style="background-color:#fffaf0;"></div>

==See Also==
*[[Aminotransferase 3D structures|Aminotransferase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermobaculum terrenum ATCC BAA-798]]
[[Category: Bezsudnova EY]]
[[Category: Boyko KM]]
[[Category: Nikolaeva AY]]
[[Category: Petrova T]]
[[Category: Popov VO]]
[[Category: Rakitina TV]]
[[Category: Suplatov DA]]
[[Category: Zeifman YS]]