View source for 7ng8


==Trimeric efflux pump Klebsiella TolC in complex with KlebC==
<StructureSection load='7ng8' size='340' side='right'caption='[[7ng8]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ng8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_quasipneumoniae Klebsiella quasipneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NG8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NG8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ng8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ng8 OCA], [https://pdbe.org/7ng8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ng8 RCSB], [https://www.ebi.ac.uk/pdbsum/7ng8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ng8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A1C3Q001_9ENTR A0A1C3Q001_9ENTR] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacteria often secrete diffusible protein toxins (bacteriocins) to kill bystander cells during interbacterial competition. Here, we use biochemical, biophysical and structural analyses to show how a bacteriocin exploits TolC, a major outer-membrane antibiotic efflux channel in Gram-negative bacteria, to transport itself across the outer membrane of target cells. Klebicin C (KlebC), a rRNase toxin produced by Klebsiella pneumoniae, binds TolC of a related species (K. quasipneumoniae) with high affinity through an N-terminal, elongated helical hairpin domain common amongst bacteriocins. The KlebC helical hairpin opens like a switchblade to bind TolC. A cryo-EM structure of this partially translocated state, at 3.1 A resolution, reveals that KlebC associates along the length of the TolC channel. Thereafter, the unstructured N-terminus of KlebC protrudes beyond the TolC iris, presenting a TonB-box sequence to the periplasm. Association with proton-motive force-linked TonB in the inner membrane drives toxin import through the channel. Finally, we demonstrate that KlebC binding to TolC blocks drug efflux from bacteria. Our results indicate that TolC, in addition to its known role in antibiotic export, can function as a protein import channel for bacteriocins.

Toxin import through the antibiotic efflux channel TolC.,Housden NG, Webby MN, Lowe ED, El-Baba TJ, Kaminska R, Redfield C, Robinson CV, Kleanthous C Nat Commun. 2021 Jul 30;12(1):4625. doi: 10.1038/s41467-021-24930-y. PMID:34330923<ref>PMID:34330923</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ng8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Klebsiella quasipneumoniae]]
[[Category: Large Structures]]
[[Category: Housden NG]]
[[Category: Kleanthous C]]
[[Category: Webby MN]]