View source for 7nie


==putative glycerol kinase-like proteins anchored on an array of voltage dependent anion channels in the outer mitochondrial membrane of pig sperm mitochondria==
<StructureSection load='7nie' size='340' side='right'caption='[[7nie]], [[Resolution|resolution]] 35.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7nie]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NIE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NIE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 35&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nie FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nie OCA], [https://pdbe.org/7nie PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nie RCSB], [https://www.ebi.ac.uk/pdbsum/7nie PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nie ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A287BD08_PIG A0A287BD08_PIG] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mitochondria-cytoskeleton interactions modulate cellular physiology by regulating mitochondrial transport, positioning, and immobilization. However, there is very little structural information defining mitochondria-cytoskeleton interfaces in any cell type. Here, we use cryofocused ion beam milling-enabled cryoelectron tomography to image mammalian sperm, where mitochondria wrap around the flagellar cytoskeleton. We find that mitochondria are tethered to their neighbors through intermitochondrial linkers and are anchored to the cytoskeleton through ordered arrays on the outer mitochondrial membrane. We use subtomogram averaging to resolve in-cell structures of these arrays from three mammalian species, revealing they are conserved across species despite variations in mitochondrial dimensions and cristae organization. We find that the arrays consist of boat-shaped particles anchored on a network of membrane pores whose arrangement and dimensions are consistent with voltage-dependent anion channels. Proteomics and in-cell cross-linking mass spectrometry suggest that the conserved arrays are composed of glycerol kinase-like proteins. Ordered supramolecular assemblies may serve to stabilize similar contact sites in other cell types in which mitochondria need to be immobilized in specific subcellular environments, such as in muscles and neurons.

In-cell structures of conserved supramolecular protein arrays at the mitochondria-cytoskeleton interface in mammalian sperm.,Leung MR, Zenezini Chiozzi R, Roelofs MC, Hevler JF, Ravi RT, Maitan P, Zhang M, Henning H, Bromfield EG, Howes SC, Gadella BM, Heck AJR, Zeev-Ben-Mordehai T Proc Natl Acad Sci U S A. 2021 Nov 9;118(45):e2110996118. doi: , 10.1073/pnas.2110996118. PMID:34737233<ref>PMID:34737233</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7nie" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Sus scrofa]]
[[Category: Leung MR]]
[[Category: Zeev-Ben-Mordehai T]]