View source for 7njh


==HEX1 (in cellulo) grown inside HARE serial crystallography chip==
<StructureSection load='7njh' size='340' side='right'caption='[[7njh]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7njh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa_OR74A Neurospora crassa OR74A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NJH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NJH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7njh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7njh OCA], [https://pdbe.org/7njh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7njh RCSB], [https://www.ebi.ac.uk/pdbsum/7njh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7njh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HEX1_NEUCR HEX1_NEUCR] Major protein in the hexagonal crystals of Woronin bodies, a peroxisomal vesicle that seal the septal pore in response to cellular damage.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Fixed-target serial crystallography has become an important method for the study of protein structure and dynamics at synchrotrons and X-ray free-electron lasers. However, sample homogeneity, consumption and the physical stress on samples remain major challenges for these high-throughput experiments, which depend on high-quality protein microcrystals. The batch crystallization procedures that are typically applied require time- and sample-intensive screening and optimization. Here, a simple protein crystallization method inside the features of the HARE serial crystallography chips is reported that circumvents batch crystallization and allows the direct transfer of canonical vapor-diffusion conditions to in-chip crystallization. Based on conventional hanging-drop vapor-diffusion experiments, the crystallization solution is distributed into the wells of the HARE chip and equilibrated against a reservoir with mother liquor. Using this simple method, high-quality microcrystals were generated with sufficient density for the structure determination of four different proteins. A new protein variant was crystallized using the protein concentrations encountered during canonical crystallization experiments, enabling structure determination from approximately 55 microg of protein. Additionally, structure determination from intracellular crystals grown in insect cells cultured directly in the features of the HARE chips is demonstrated. In cellulo crystallization represents a comparatively unexplored space in crystallization, especially for proteins that are resistant to crystallization using conventional techniques, and eliminates any need for laborious protein purification. This in-chip technique avoids harvesting the sensitive crystals or any further physical handling of the crystal-containing cells. These proof-of-principle experiments indicate the potential of this method to become a simple alternative to batch crystallization approaches and also as a convenient extension to canonical crystallization screens.

A simple vapor-diffusion method enables protein crystallization inside the HARE serial crystallography chip.,Norton-Baker B, Mehrabi P, Boger J, Schonherr R, von Stetten D, Schikora H, Kwok AO, Martin RW, Miller RJD, Redecke L, Schulz EC Acta Crystallogr D Struct Biol. 2021 Jun 1;77(Pt 6):820-834. doi:, 10.1107/S2059798321003855. Epub 2021 May 19. PMID:34076595<ref>PMID:34076595</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7njh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Neurospora crassa OR74A]]
[[Category: Boger J]]
[[Category: Martin RW]]
[[Category: Mehrabi P]]
[[Category: Miller RJD]]
[[Category: Norton-Baker B]]
[[Category: Redecke L]]
[[Category: Schikora H]]
[[Category: Schonherr R]]
[[Category: Schulz EC]]
[[Category: Von Stetten D]]