View source for 7ocy


==Enterococcus faecalis EfrCD in complex with a nanobody==
<StructureSection load='7ocy' size='340' side='right'caption='[[7ocy]], [[Resolution|resolution]] 4.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ocy]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis] and [https://en.wikipedia.org/wiki/Vicugna_pacos Vicugna pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OCY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.25&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ocy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ocy OCA], [https://pdbe.org/7ocy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ocy RCSB], [https://www.ebi.ac.uk/pdbsum/7ocy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ocy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q837P5_ENTFA Q837P5_ENTFA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Drug efflux is a common resistance mechanism found in bacteria and cancer cells, but studies providing comprehensive functional insights are scarce. In this study, we performed deep mutational scanning (DMS) on the bacterial ABC transporter EfrCD to determine the drug efflux activity profile of more than 1,430 single variants. These systematic measurements revealed that the introduction of negative charges at different locations within the large substrate binding pocket results in strongly increased efflux activity toward positively charged ethidium, whereas additional aromatic residues did not display the same effect. Data analysis in the context of an inward-facing cryogenic electron microscopy structure of EfrCD uncovered a high-affinity binding site, which releases bound drugs through a peristaltic transport mechanism as the transporter transits to its outward-facing conformation. Finally, we identified substitutions resulting in rapid Hoechst influx without affecting the efflux activity for ethidium and daunorubicin. Hence, single mutations can convert EfrCD into a drug-specific ABC importer.

Deep mutational scan of a drug efflux pump reveals its structure-function landscape.,Meier G, Thavarasah S, Ehrenbolger K, Hutter CAJ, Hurlimann LM, Barandun J, Seeger MA Nat Chem Biol. 2023 Apr;19(4):440-450. doi: 10.1038/s41589-022-01205-1. Epub 2022 , Nov 28. PMID:36443574<ref>PMID:36443574</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ocy" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Enterococcus faecalis]]
[[Category: Large Structures]]
[[Category: Vicugna pacos]]
[[Category: Barandun J]]
[[Category: Ehrenbolger K]]
[[Category: Hutter CAJ]]
[[Category: Meier G]]
[[Category: Seeger MA]]